| Comparison of the NucliSens easyMAG and Qiagen BioRobot 9604 nucleic acid extraction systems for detection of RNA and DNA respiratory viruses in nasopharyngeal aspirate samples. | |
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MedLine Citation:
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PMID: 18463206 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The NucliSens easyMAG and BioRot 9604 automated nucleic acid extraction systems were evaluated and compared with the manual QIAamp (Qiagen) extraction method for their abilities to extract nucleic acid from nasopharyngeal aspirate samples for the detection of RNA and DNA respiratory viruses. The nucleic acids recovered by all three methods gave comparable sensitivities in PCR tests, and the three methods gave comparable viral loads. There was no evidence of residual PCR inhibitors and no evidence of PCR cross-contamination. |
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Authors:
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Kwok Hung Chan; Wing Cheong Yam; Chiu Mei Pang; Kit Man Chan; Siu Yan Lam; Kam Fai Lo; Leo L M Poon; J S Malik Peiris |
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Publication Detail:
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Type: Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't Date: 2008-05-07 |
Journal Detail:
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Title: Journal of clinical microbiology Volume: 46 ISSN: 1098-660X ISO Abbreviation: J. Clin. Microbiol. Publication Date: 2008 Jul |
Date Detail:
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Created Date: 2008-07-10 Completed Date: 2008-08-12 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505564 Medline TA: J Clin Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 2195-9 Citation Subset: IM |
Affiliation:
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Department of Microbiology, University Pathology Building, Queen Mary Hospital Compound, Pokfulam, Hong Kong, SAR, People's Republic of China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Automation DNA Viruses / isolation & purification* DNA, Viral / isolation & purification* Hong Kong Hospitals Humans Nasopharynx / virology* Polymerase Chain Reaction / methods RNA Viruses / isolation & purification* RNA, Viral / isolation & purification* Sensitivity and Specificity Virology / methods* Virus Diseases / diagnosis* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Viral; 0/RNA, Viral |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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