Document Detail

Comparison of neutrophil apoptosis, α-defensins and calprotectin in children with and without severe early childhood caries.
MedLine Citation:
PMID:  21427491     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: The infectious nature of severe early-childhood caries (S-ECC) points to the possible participation of immunologic host responses including neutrophils and their antimicrobial products.
OBJECTIVES: The aim of this study was to determine the neutrophil apoptosis, α-defensins (HNP1-3) and calprotectin levels in the saliva of preschool children and the association with S-ECC.
METHODS: Oral examinations were performed on 87 children aged 3-5 years and non stimulated whole saliva samples were collected. Thirty of these subjects were considered S-ECC children, 30 with moderate caries (MC) and 27 were caries free (CF). To detect apoptosis, cell staining was done with Annexin-V-Fluos and propidium iodide, and they were analyzed by fluorescent microscopy. The concentration of α-defensins and calprotectin were assessed using ELISA.
RESULTS: There were no statistical differences between groups considering the HNP1-3 or calprotectin salivary levels (p=0.06 and p=0.23, respectively). The HNP1-3 and calprotectin levels were negatively correlated and the correlation was significant in MC group (p=0.03). Lower levels of apoptotic neutrophils were obtained from CF subjects as compared with S-ECC children (p=0.03).
CONCLUSIONS: Our findings establish that apoptotic mechanisms could be implicated in the immunity responses associated with S-ECC. We cannot yet determine if the level of salivary α-defensins or calprotectin is predictive of S-ECC.
Lida Toomarian; Mandana Sattari; Nazanin Hashemi; Nikoo Tadayon; Alireza Akbarzadeh Baghban
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Iranian journal of immunology : IJI     Volume:  8     ISSN:  1735-1383     ISO Abbreviation:  Iran J Immunol     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-03-23     Completed Date:  2011-09-01     Revised Date:  2014-07-30    
Medline Journal Info:
Nlm Unique ID:  101282932     Medline TA:  Iran J Immunol     Country:  Iran    
Other Details:
Languages:  eng     Pagination:  11-9     Citation Subset:  IM    
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MeSH Terms
Apoptosis / immunology*
Child, Preschool
Dental Caries / immunology*
Leukocyte L1 Antigen Complex / chemistry,  immunology*
Neutrophils / cytology,  immunology*
Saliva / chemistry,  cytology,  immunology*
alpha-Defensins / chemistry,  immunology*
Reg. No./Substance:
0/Leukocyte L1 Antigen Complex; 0/alpha-Defensins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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