| Comparison of Neutrophil Apoptosis, α-Defensins and Calprotectin in Children with and without Severe Early Childhood Caries. | |
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MedLine Citation:
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PMID: 21427491 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Background: The infectious nature of severe early-childhood caries (S-ECC) points to the possible participation of immunologic host responses including neutrophils and their antimicrobial products. Objectives: The aim of this study was to determine the neutrophil apoptosis, α-defensins (HNP1-3) and calprotectin levels in the saliva of preschool children and the association with S-ECC. Methods: Oral examinations were performed on 87 children aged 3-5 years and non stimulated whole saliva samples were collected. Thirty of these subjects were considered S-ECC children, 30 with moderate caries (MC) and 27 were caries free (CF). To detect apoptosis, cell staining was done with Annexin-V-Fluos and propidium iodide, and they were analyzed by fluorescent microscopy. The concentration of α-defensins and calprotectin were assessed using ELISA. Results: There were no statistical differences between groups considering the HNP1-3 or calprotectin salivary levels (p=0.06 and p=0.23, respectively). The HNP1-3 and calprotectin levels were negatively correlated and the correlation was significant in MC group (p=0.03). Lower levels of apoptotic neutrophils were obtained from CF subjects as compared with S-ECC children (p=0.03). Conclusions: Our findings establish that apoptotic mechanisms could be implicated in the immunity responses associated with S-ECC. We cannot yet determine if the level of salivary α-defensins or calprotectin is predictive of S-ECC. |
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Authors:
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Lida Toomarian; Mandana Sattari; Nazanin Hashemi; Nikoo Tadayon; Alireza Akbarzadeh Baghban |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Iranian journal of immunology : IJI Volume: 8 ISSN: 1735-1383 ISO Abbreviation: Iran J Immunol Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-03-23 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101282932 Medline TA: Iran J Immunol Country: Iran |
Other Details:
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Languages: eng Pagination: 11-9 Citation Subset: IM |
Affiliation:
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Department of Pediatric Dentistry, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran, e-mail: ltoomarian@yahoo.com. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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