Document Detail

Comparison of Neutrophil Apoptosis, α-Defensins and Calprotectin in Children with and without Severe Early Childhood Caries.
MedLine Citation:
PMID:  21427491     Owner:  NLM     Status:  In-Data-Review    
Background: The infectious nature of severe early-childhood caries (S-ECC) points to the possible participation of immunologic host responses including neutrophils and their antimicrobial products. Objectives: The aim of this study was to determine the neutrophil apoptosis, α-defensins (HNP1-3) and calprotectin levels in the saliva of preschool children and the association with S-ECC. Methods: Oral examinations were performed on 87 children aged 3-5 years and non stimulated whole saliva samples were collected. Thirty of these subjects were considered S-ECC children, 30 with moderate caries (MC) and 27 were caries free (CF). To detect apoptosis, cell staining was done with Annexin-V-Fluos and propidium iodide, and they were analyzed by fluorescent microscopy. The concentration of α-defensins and calprotectin were assessed using ELISA. Results: There were no statistical differences between groups considering the HNP1-3 or calprotectin salivary levels (p=0.06 and p=0.23, respectively). The HNP1-3 and calprotectin levels were negatively correlated and the correlation was significant in MC group (p=0.03). Lower levels of apoptotic neutrophils were obtained from CF subjects as compared with S-ECC children (p=0.03). Conclusions: Our findings establish that apoptotic mechanisms could be implicated in the immunity responses associated with S-ECC. We cannot yet determine if the level of salivary α-defensins or calprotectin is predictive of S-ECC.
Lida Toomarian; Mandana Sattari; Nazanin Hashemi; Nikoo Tadayon; Alireza Akbarzadeh Baghban
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Iranian journal of immunology : IJI     Volume:  8     ISSN:  1735-1383     ISO Abbreviation:  Iran J Immunol     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-03-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101282932     Medline TA:  Iran J Immunol     Country:  Iran    
Other Details:
Languages:  eng     Pagination:  11-9     Citation Subset:  IM    
Department of Pediatric Dentistry, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran, e-mail:
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