Document Detail

Comparison of nasal and nasopharyngeal swabs for influenza detection in adults.
MedLine Citation:
PMID:  22723469     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: Examine differences in the detection of influenza by specimen and test type using paired nasal and nasopharyngeal swabs.
DESIGN: Prospective study
SETTING: Enrollment took place between January and March 2007 in a central Wisconsin population.
PARTICIPANTS: Adult patients were screened and enrolled by trained research coordinators following medical encounters for acute respiratory illnesses of <10 days duration.
METHODS: Paired nasal and nasopharyngeal swabs were collected from consenting patients and tested by both real-time reverse transcriptase polymerase chain reaction (rRT-PCR) and viral culture. A composite measure of positivity was used as the gold standard; cases included any positive result by rRT-PCR or viral culture from either specimen type.
RESULTS: Paired samples were collected from 240 adults; 33 (14%) individuals tested positive for influenza by rRT-PCR. Using rRT-PCR, the sensitivity of the nasal swab was 89% (95% CI, 78%-99%) and the sensitivity of the nasopharyngeal swab was 94% (95% CI, 87%-100%), compared to a composite gold standard.
CONCLUSION: Test sensitivity did not vary significantly by swab type when using a highly sensitive molecular diagnostic test, but power was limited to detect modest differences.
Stephanie A Irving; Mary F Vandermause; David K Shay; Edward A Belongia
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Publication Detail:
Type:  Comparative Study; Controlled Clinical Trial; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S.     Date:  2012-06-21
Journal Detail:
Title:  Clinical medicine & research     Volume:  10     ISSN:  1554-6179     ISO Abbreviation:  Clin Med Res     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-12     Completed Date:  2013-04-25     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  101175887     Medline TA:  Clin Med Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  215-8     Citation Subset:  IM    
Epidemiology Research Center, Marshfield Clinic Research Foundation, Marshfield, WI, USA.
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MeSH Terms
Aged, 80 and over
Confidence Intervals
Influenza A virus / isolation & purification*
Influenza B virus / isolation & purification*
Influenza, Human / diagnosis*
Middle Aged
Nasopharynx / virology*
Nose / virology*
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Sensitivity and Specificity
Specimen Handling / methods*
Virology / methods
Grant Support
1 U01 CI000192-01/CI/NCPDCID CDC HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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