Document Detail


Comparison of nanotube-protein corona composition in cell culture media.
MedLine Citation:
PMID:  23322550     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In biological environments, nanomaterials associate with proteins forming a protein corona (PC). The PC may alter the nanomaterial's pharmacokinetics and pharmacodynamics, thereby influencing toxicity. Using a label-free mass spectrometry-based proteomics approach, the composition of the PC is examined for a set of nanotubes (NTs) including unmodified and carboxylated single- (SWCNT) and multi-walled carbon nanotubes (MWCNT), polyvinylpyrrolidone (PVP)-coated MWCNT (MWCNT-PVP), and nanoclay. NTs are incubated for 1 h in simulated cell culture conditions, then washed, resuspended in PBS, and assessed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) for their associated PC. To determine those attributes that influence PC formation, the NTs are extensively characterized. NTs had negative zeta potentials in water (SWCNT-COOH < MWCNT-COOH < unmodified NTs) while carboxylation increases their hydrodynamic sizes. All NTs are also found to associate a common subset of proteins including albumin, titin, and apolipoproteins. SWCNT-COOH and MWCNT-COOH are found to bind the greatest number of proteins (181 and 133 respectively) compared to unmodified NTs (<100), suggesting covalent binding to protein amines. Modified NTs bind a number of unique proteins compared to unmodified NTs, implying hydrogen bonding and electrostatic interactions are involved in PC formation. PVP-coating of MWCNT did not influence PC composition, further reinforcing the possibility of hydrogen bonding and electrostatic interactions. No relationships are found between PC composition and corresponding isoelectric point, hydropathy, or aliphatic index, implying minimal roles of hydrophobic interaction and pi-stacking.
Authors:
Jonathan H Shannahan; Jared M Brown; Ran Chen; Pu Chun Ke; Xianyin Lai; Somenath Mitra; Frank A Witzmann
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2013-01-16
Journal Detail:
Title:  Small (Weinheim an der Bergstrasse, Germany)     Volume:  9     ISSN:  1613-6829     ISO Abbreviation:  Small     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-06-14     Completed Date:  2014-01-13     Revised Date:  2014-04-14    
Medline Journal Info:
Nlm Unique ID:  101235338     Medline TA:  Small     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  2171-81     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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MeSH Terms
Descriptor/Qualifier:
Culture Media
Mass Spectrometry
Nanotubes, Carbon / chemistry*
Povidone / chemistry
Proteins / chemistry*
Proteomics
Grant Support
ID/Acronym/Agency:
R01 ES019311/ES/NIEHS NIH HHS; R01 ES019311/ES/NIEHS NIH HHS; R01 GM085218/GM/NIGMS NIH HHS; R01GM085218/GM/NIGMS NIH HHS; RC2 ES018025/ES/NIEHS NIH HHS; RC2 ES018810/ES/NIEHS NIH HHS; U19 ES019525/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Culture Media; 0/Nanotubes, Carbon; 0/Proteins; 9003-39-8/Povidone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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