Document Detail


Comparison of Lumin LED fluorescent attachment, fluorescent microscopy and Ziehl-Neelsen for AFB diagnosis.
MedLine Citation:
PMID:  19555532     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
DESIGN: Cross-sectional studies in Russia (n = 502) and Macedonia (n = 205), with fluorochrome-stained sputum examined by 1) the new Lumin light emitting diode (LED) fluorescent attachment on a light microscope, and 2) conventional fluorescent microscope (CFM) available in each laboratory, and compared to 3) Ziehl-Neelsen (ZN) restaining/reading of the same smears. Poor readings of ZN-restained smears in Russia stimulated a retrospective laboratory registry analysis for sensitivity and specificity of directly ZN-stained smears (n = 791) from a previous period. RESULTS: In Macedonia, the sensitivity of the Lumin and CFM were 87.8%, and that of restained ZN smears with conventional light microscope was 78.0%. In Russia, sensitivity was as follows: Lumin 72.8%, CFM 52.5%; re-stained ZN smears 28.5% and directly ZN stained smears 55.6%. CONCLUSION: Fluorescence microscopy is more sensitive than conventional microscopy. The Lumin attachment to conventional light microscopes provided results equal to or better than the CFMs. Smear restaining for ZN showed a 12% advantage for Lumin and CFM in Macedonia, in line with other meta-analyses. Restaining for ZN gave poor results in Russia for unknown reasons. Retrospective analysis of directly ZN-stained smears showed 55.6% sensitivity compared to the Lumin (72.8%), which is also in line with the superiority of fluorescent microscopy reported in literature.
Authors:
Alexander Trusov; R Bumgarner; R Valijev; R Chestnova; S Talevski; C Vragoterova; E S Neeley
Publication Detail:
Type:  Comparative Study; Journal Article; Multicenter Study    
Journal Detail:
Title:  The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease     Volume:  13     ISSN:  1027-3719     ISO Abbreviation:  Int. J. Tuberc. Lung Dis.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-06-26     Completed Date:  2009-09-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706389     Medline TA:  Int J Tuberc Lung Dis     Country:  France    
Other Details:
Languages:  eng     Pagination:  836-41     Citation Subset:  IM    
Affiliation:
alexandertrusov@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Benzophenoneidum / diagnostic use*
Colony Count, Microbial
Coloring Agents / diagnostic use*
Cross-Sectional Studies
Humans
Macedonia (Republic)
Microscopy, Fluorescence / instrumentation*
Prospective Studies
Russia
Semiconductors*
Sensitivity and Specificity
Sputum / microbiology*
Tuberculosis, Pulmonary / diagnosis*
Chemical
Reg. No./Substance:
0/Coloring Agents; 2465-27-2/Benzophenoneidum

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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