Document Detail

Comparison of low-abundance biomarker levels in capillary-collected nonstimulated tears and washout tears of aqueous-deficient and normal patients.
MedLine Citation:
PMID:  23611994     Owner:  NLM     Status:  MEDLINE    
PURPOSE: Low tear volume limits the use of nonstimulated (NS) microcapillary tear collection in aqueous-deficient (AD) patients. Adding a small amount of "washout" fluid to the eye prior to tear collection is a potentially viable alternative method for abundant proteins, but is relatively untested for low-abundance biomarkers. This study determined the feasibility of the washout (WO) method as an NS alternative for low-abundance biomarkers. NS and WO biomarker profiles were compared between AD patients and non-AD controls to determine if the two methods identify the same intergroup differences.
METHODS: Matching NS and WO tears were collected from 48 patients by micropipette, the WO sample after instillation of 10 μL saline. Tear cytokine levels were measured by 27-Plex Bio-Rad assay. Bland-Altman analyses for each biomarker determined the agreement between tear sample types. Patients were grouped as AD or non-AD based on Schirmer score to determine if NS profile between-group differences were preserved in WO tears.
RESULTS: Bland-Altman plots showed good biomarker level agreement between NS and WO tears for most cytokines. Five biomarkers, among those most often cited as differing in AD dry eye, differed significantly between non-AD and AD groups in both tear types. Additional biomarker differences were seen in NS tears only.
CONCLUSIONS: The WO tear collection method is a viable alternative to NS tears for many low-abundance biomarkers and is able to replicate major NS tear differences between dry eye groups. More subtle intergroup differences are lost in WO samples because of reduced statistical power.
Nicole Guyette; Larezia Williams; My-Tho Tran; Tammy Than; John Bradley; Lucy Kehinde; Clara Edwards; Mark Beasley; Roderick Fullard
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Publication Detail:
Type:  Clinical Trial; Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-05-01
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  54     ISSN:  1552-5783     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-06-24     Completed Date:  2013-09-06     Revised Date:  2013-11-05    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3729-37     Citation Subset:  IM    
Department of Vision Sciences, School of Optometry, University of Alabama-Birmingham, Birmingham, AL 35294, USA.
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MeSH Terms
Biological Markers / metabolism
Capillary Action*
Cytokines / metabolism*
Dry Eye Syndromes / metabolism*
Eye Proteins / metabolism
Feasibility Studies
Middle Aged
Sodium Chloride / administration & dosage*
Specimen Handling / methods
Tears / metabolism*
Young Adult
Grant Support
Reg. No./Substance:
0/Biological Markers; 0/Cytokines; 0/Eye Proteins; 7647-14-5/Sodium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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