Document Detail


Comparison of lipid and fatty acid composition of the liver, subcutaneous and intra-abdominal adipose tissue, and serum.
MedLine Citation:
PMID:  19798063     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ceramides may mediate saturated fat-induced insulin resistance, but there are no data comparing ceramide concentrations between human tissues. We therefore performed lipidomic analysis of human subcutaneous (SCfat) and intra-abdominal (IAfat) adipose tissue, the liver, and serum in eight subjects. The liver contained (nmol/mg tissue) significantly more ceramides (1.5-3-fold), sphingomyelins (7-8-fold), phosphatidylethanolamines (10-11-fold), lysophosphatidylcholines (7-12-fold), less ether-linked phosphatidylcholines (2-2.5-fold) but similar amounts of diacylglycerols as compared to SCfat and IAfat. The amounts of ceramides and their synthetic precursors, such as palmitic (16:0) free fatty acids and sphingomyelins, differed considerably between the tissues. The liver contained proportionally more palmitic, stearic (18:0), and long polyunsaturated fatty acids than adipose tissues. Stearoyl-CoA desaturase 1 (SCD1) activity reflected by serum, estimated from the 16:1/16:0-ratio, was closely related to that in the liver (r = 0.86, P = 0.024) but not adipose tissues. This was also true for estimated elongase (18:1/16:1, r = 0.89, P = 0.01), and Delta5 (20:4/20:3, r = 0.89, P = 0.012) and Delta6 (18:3[n-6]/18:2, r = 1.0, P < 0.001) desaturase activities. We conclude that the human liver contains higher concentrations of ceramides and saturated free fatty acids than either SCfat or IAfat.
Authors:
Anna Kotronen; Tuulikki Seppänen-Laakso; Jukka Westerbacka; Tuula Kiviluoto; Johanna Arola; Anna-Liisa Ruskeepää; Hannele Yki-Järvinen; Matej Oresic
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-01
Journal Detail:
Title:  Obesity (Silver Spring, Md.)     Volume:  18     ISSN:  1930-739X     ISO Abbreviation:  Obesity (Silver Spring)     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-28     Completed Date:  2010-07-21     Revised Date:  2012-08-13    
Medline Journal Info:
Nlm Unique ID:  101264860     Medline TA:  Obesity (Silver Spring)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  937-44     Citation Subset:  IM    
Affiliation:
Department of Medicine, Division of Diabetes, Helsinki, Finland. anna.kotronen@helsinki.fi
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MeSH Terms
Descriptor/Qualifier:
Adult
Analysis of Variance
Female
Humans
Intra-Abdominal Fat / chemistry*
Lipids / analysis*,  blood*
Liver / chemistry*
Male
Middle Aged
Patient Selection
Subcutaneous Fat / chemistry*
Chemical
Reg. No./Substance:
0/Lipids

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