Document Detail

Comparison of in vivo efficacy of different ocular lubricants in dry eye animal models.
MedLine Citation:
PMID:  24781937     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To compare the efficacy of three types of ocular lubricants in protecting corneal epithelial cells in dry eye animal models.
METHODS: Ocular lubricants containing 0.1% or 0.3% sodium hyaluronate (SH), carboxymethylcellulose (CMC), or hydroxypropyl methylcellulose (HPMC) were tested. First, ocular lubricant containing 0.002% fluorescein was dropped onto the rabbit corneas. The fluorescein intensity as an index of retention was measured. Second, a rabbit dry eye model was made by holding the eye open with a speculum, and 50 μL of each ocular lubricant was dropped onto the cornea. After 3 hours, the corneas were stained with 1% methylene blue (MB), and the absorbance of MB was measured. Third, a rat dry eye model was treated with the ocular lubricants for 4 weeks, and the corneal fluorescein staining was scored. Eyes treated with physiological saline were used as controls. Finally, immunohistochemistry was used to analyze occludin, an epithelial barrier protein, in cultured human corneal epithelial cells pretreated with ocular lubricants and desiccated for 20 or 60 minutes.
RESULTS: Our results showed that 0.3% SH had a significantly longer retention time than the other lubricants (all P < 0.01). The absorbance of MB was significantly lower in the 0.3% SH group. The corneas of rats exposed to 0.3% SH had significantly lower fluorescein staining scores. A significantly higher number of occludin-positive cells were found after exposure to 0.3% SH than other lubricants.
CONCLUSIONS: Ocular lubricant containing 0.3% SH would be preferable to treat patients with dry eye syndrome.
Xiaodong Zheng; Tomoko Goto; Yuichi Ohashi
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2014-04-29
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  55     ISSN:  1552-5783     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  2014 Jun 
Date Detail:
Created Date:  2014-06-06     Completed Date:  2014-07-29     Revised Date:  2014-08-19    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3454-60     Citation Subset:  IM    
Copyright Information:
Copyright 2014 The Association for Research in Vision and Ophthalmology, Inc.
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MeSH Terms
Carboxymethylcellulose Sodium / administration & dosage*
Cornea / drug effects*
Disease Models, Animal
Dry Eye Syndromes / drug therapy*
Hyaluronic Acid / administration & dosage*
Methylcellulose / administration & dosage,  analogs & derivatives*
Ophthalmic Solutions / administration & dosage
Treatment Outcome
Viscosupplements / administration & dosage
Reg. No./Substance:
0/Ophthalmic Solutions; 0/Viscosupplements; 8063-82-9/hypromellose; 9004-32-4/Carboxymethylcellulose Sodium; 9004-61-9/Hyaluronic Acid; 9004-67-5/Methylcellulose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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