| Comparison of Hybrid Capture 2, Linear Array and a bead-based multiplex genotyping assay for the detection of Human Papillomavirus in negative Pap tests and Atypical Squamous Cells of Undetermined Significance. | |
| | |
MedLine Citation:
|
PMID: 23035194 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Background. Many methods with different analytical sensitivity and clinical specificity have been developed to detect the presence of high-risk (HR) types of the human papillomavirus (HPV) in cervical samples. The Hybrid Capture-II assay (HC-II) is broadly used for primary screening. In addition, several HPV genotyping assays, based on polymerase chain reaction (PCR) methods, display a higher sensitivity than HC-II and are also used in screening programs.Method. We evaluated the performance of three HPV DNA tests, namely HC II, Linear Array HPV genotyping assay (LA) and a bead-based multiplex genotyping assay (TS-MPG) which is a laboratory-developed method for the detection of HPV in 94 women with ASC-US and in cytological samples from 86 women with a negative Pap test.Results. The TS-MPG assay increased the HPV prevalence compared to LA and HC-II. The HPV DNA prevalence was greater for ASC-US with the TS-MPG assay (46.2%), compared to LA (36.3%) and HC-II (29.7%) assays.The HPV DNA prevalence was greater for the control group with the TS-MPG assay (32.1%), compared to the LA assay (10.7%).Two ASC-US women who were HPV DNA negative by HC-II and positive by TS-MPG or/and LA assays, had lesions that progressed to low-grade squamous intraepithelial and high- grade squamous intraepithelial lesions.Conclusion. This study shows that the TS-MPG assay exhibited higher analytical sensitivity compared to LA and HC-II for the detection of HPV DNA, which reduces the potential to incorrectly identify a woman's HPV infection status. |
| | |
Authors:
|
Manola Comar; Michelle Iannacone; Giorgia Casalicchio; Sandrine McKay-Chopin; Massimo Tommasino; Tarik Gheit |
Related Documents
:
|
16011734 - Cytotoxicity of abnormal savda munziq aqueous extract in human hepatoma (hepg2) cells. 3827244 - Dynamics of extracellular dna in the marine environment. 23385824 - Excised radicle tips as a source of genomic dna for pcr-based genotyping and melting cu... 3010854 - Incorporation of 1-beta-d-arabinofuranosylcytosine into dna and mutagenesis of herpes s... 9349824 - Crystallographic structure of the t domain-dna complex of the brachyury transcription f... 20532374 - Blunt-ended dna stacking interactions in a 3-helix motif. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-10-3 |
Journal Detail:
|
Title: Journal of clinical microbiology Volume: - ISSN: 1098-660X ISO Abbreviation: J. Clin. Microbiol. Publication Date: 2012 Oct |
Date Detail:
|
Created Date: 2012-10-4 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7505564 Medline TA: J Clin Microbiol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Institute for Maternal and Child Health - IRCCS "Burlo Garofolo", Via dell'Istria 65/1, 34137 Trieste, Italy. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Characterisation of a verocytotoxin-producing enteroaggregative Escherichia coli serogroup O111:H21 ...
Next Document: Atypical H-Type Bovine Spongiform Encephalopathy in a Cow Born after the Reinforced Feed-Ban on Meat...