Document Detail


Comparison of HeLa-I, HEp-2 and NCI-H292 cell lines for the isolation of human respiratory syncytial virus (HRSV).
MedLine Citation:
PMID:  17825929     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Generally, laboratory diagnosis of viral respiratory infections utilizes virus isolation in cell culture and immunofluorescence assays. In this study, three cell lines (HEp-2, NCI-H292 and HeLa-I) were used for HRSV isolation of strains obtained from patients admitted at HU-USP with respiratory tract disease. HRSV was isolated in 46% (37) of 80 specimens inoculated in HeLa-I, 48% (39) in HEp-2, and 36.3% (29) in NCI-H292. Immunofluorescence was considered the gold standard and yielded 53% positive (43). The results from both methods combined had better sensitivity (73.2%) compared to either method alone. Comparing results between the cell lines with HEp-2 cells as the benchmark, the greatest sensitivity (72.2%) was observed in HeLa-I. This data shows that HeLa-I is adequate for HRSV isolation, giving results similar to the HEp-2 cells. The combined use of the HEp-2, HeLa-I and NCI-H292 cells improve the detection of HRSV.
Authors:
Ana Priscila Perini; Maria Luisa Barbosa; Viviane Fongaro Botosso; Claudia Trigo Pedroso de Moraes; Alfredo E Gillio; Noeli Hens; Klaus E Stewien; Edison L Durigon
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2007-09-07
Journal Detail:
Title:  Journal of virological methods     Volume:  146     ISSN:  0166-0934     ISO Abbreviation:  J. Virol. Methods     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-11-07     Completed Date:  2008-01-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8005839     Medline TA:  J Virol Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  368-71     Citation Subset:  IM    
Affiliation:
Universidade de São Paulo, Instituto de Ciências Biomédicas, São Paulo, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Cell Line, Tumor
Cytopathogenic Effect, Viral
Fluorescent Antibody Technique, Indirect
Hela Cells
Humans
Infant
Nasopharynx / virology
Respiratory Syncytial Virus Infections / diagnosis,  virology
Respiratory Syncytial Virus, Human / isolation & purification*,  physiology
Sensitivity and Specificity
Virus Cultivation

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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