Document Detail

Comparison of fresh corneal tissue versus glycerin-cryopreserved corneal tissue in deep anterior lamellar keratoplasty.
MedLine Citation:
PMID:  19737874     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To compare the microstructural differences in fresh corneal tissue (FCT) with glycerin-cryopreserved corneal tissue (GCCT) used during deep anterior lamellar keratoplasty (DALK). METHODS: The medical records of 48 consecutive patients who underwent DALK for stromal opacity without endothelial abnormalities were retrospectively reviewed. Patients were divided into two groups: an FCT group (n = 22) and a GCCT group (n = 26), according to the corneal tissue used. The best corrected visual acuity (BCVA), slit lamp, corneal topography, pachymetry, and laser scanning in vivo confocal microscopy examinations at 2 weeks and 1, 3, 6, 12, and 24 months after surgery were analyzed. RESULTS: No graft rejection developed in the GCCT group, whereas stromal rejection developed in one eye in the FCT group. There were no significant differences in spherical equivalent (P = 0.37), astigmatism (P = 0.26), BCVA (P = 0.64), central corneal thickness (P = 0.73), or endothelial cell density (P = 0.49) between the two groups at 24 months. Confocal microscopy showed that GCCT was acellular, whereas dendritelike cells and keratocytes were found in the FCT group 2 weeks after surgery. The keratocyte density improved significantly in the GCCT group at 3 months after surgery, whereas it decreased significantly after surgery in the FCT group during follow-up. No significant difference in regeneration of nerve fibers was found in the subbasal layer and anterior stroma between the two groups at 24 months. CONCLUSIONS: DALK using GCCT provides clinical results comparable to FCT. GCCT can be used safely and effectively for DALK and may minimize stromal rejection after surgery.
Wei Chen; Ying Lin; Xiaobo Zhang; Lihua Wang; Mimi Liu; Jingbo Liu; Ya Ye; Li Sun; Huixiang Ma; Jia Qu
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-09-08
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  51     ISSN:  1552-5783     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-01-19     Completed Date:  2010-02-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  775-81     Citation Subset:  IM    
School of Ophthalmology and Optometry, Wenzhou Medical College, Zhejiang, China.
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MeSH Terms
Cell Count
Corneal Opacity / surgery*
Corneal Stroma / pathology
Corneal Topography
Corneal Transplantation / methods*
Cryoprotective Agents / therapeutic use*
Endothelium, Corneal / cytology
Glycerol / therapeutic use*
Graft Survival / physiology
Microscopy, Confocal
Middle Aged
Refraction, Ocular / physiology
Retrospective Studies
Tissue Donors
Tomography, Optical Coherence
Visual Acuity / physiology
Young Adult
Reg. No./Substance:
0/Cryoprotective Agents; 56-81-5/Glycerol

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