Document Detail

Comparison of Flow Cytometry and Indirect Immunofluorescence Assay in the Diagnosis and Cure Criterion after Therapy of American Tegumentary Leishmaniasis by Anti-live Leishmania (Viannia) braziliensis Immunoglobulin G.
MedLine Citation:
PMID:  23142460     Owner:  NLM     Status:  Publisher    
The aim of this study was to compare the techniques of indirect immunofluorescence assay (IFA) and flow cytometry to clinical and laboratorial evaluation of patients before and after clinical cure and to evaluate the applicability of flow cytometry in post-therapeutic monitoring of patients with american tegumentary leishmaniasis (ATL). Sera from 14 patients before treatment (BT), 13 patients 1 year after treatment (AT), 10 patients 2 and 5 years AT were evaluated. The results from flow cytometry were expressed as levels of IgG reactivity, based on the percentage of positive fluorescent parasites (PPFP). The 1:256 sample dilution allowed us to differentiate individuals BT and AT. Comparative analysis of IFA and flow cytometry by ROC (Receiver Operating Characteristic Curve) showed, respectively, AUC (area under curve) = 0.8 (95% CI = 0.64-0.89) and AUC = 0,90 (95% CI = 0,75-0.95), demonstrating that the flow cytometry had equivalent accuracy. Our data demonstrated that 20% was the best cut-off point identified by the ROC curve for the flow cytometry assay. This test showed a sensitivity of 86% and specificity of 77% while the IFA had a sensitivity of 78% and specificity of 85%. The after-treatment screening, through comparative analysis of the techniques performance indexes, 1, 2 and 5 years AT, showed an equal performance of the flow cytometry comparing with the IFA. However, flow cytometry shows to be a better diagnostic alternative when applied to the study of ATL in the cure criterion. The information obtained in this work opens perspectives to monitor cure after treatment of ATL.
Andresa Pereira de Oliveira; Maria Carolina Accioly Brelaz de Castro; Amanda Ferreira de Almeida; Marina de Assis Souza; Beatriz Coutinho de Oliveira; Luiza Campos Reis; Hiro Goto; Maria Edileuza Felinto de Brito; Beatriz Julieta Celeste; Olindo Assis Martins-Filho; Valéria Rêgo Alves Pereira
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-8
Journal Detail:
Title:  Journal of immunological methods     Volume:  -     ISSN:  1872-7905     ISO Abbreviation:  J. Immunol. Methods     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1305440     Medline TA:  J Immunol Methods     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier B.V.
Centro de Pesquisas Aggeu Magalhães, FIOCRUZ, Recife, Pernambuco, Brazil. Electronic address:
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