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Comparison of enzymatic and non-enzymatic means of dissociating adherent monolayers of mesenchymal stem cells.
MedLine Citation:
PMID:  19495917     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
The dissociation of adherent mesenchymal stem cell (MSC) monolayers with trypsin and enzyme-free dissociation buffer was compared. A significantly lower proportion of viable cells were obtained with enzyme-free dissociation buffers compared to trypsin. Subsequently, the dissociated cells were re-seeded on new cell culture dishes and were subjected to the MTT assay 24 h later. The proportion of viable cells that reattached was significantly lower for cells obtained by dissociation with enzyme-free dissociation buffer compared to trypsin. Frozen-thawed MSC displayed a similar trend, yielding consistently higher cell viability and reattachment rates when dissociated with trypsin compared to enzyme-free dissociation buffer. It was also demonstrated that exposure of trypsin-dissociated MSC to enzyme-free dissociation buffer for 1 h had no significant detrimental effect on cell viability.
Authors:
Boon C Heng; Catherine M Cowan; Shubhayu Basu
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Publication Detail:
Type:  Journal Article     Date:  2009-03-10
Journal Detail:
Title:  Biological procedures online     Volume:  11     ISSN:  1480-9222     ISO Abbreviation:  Biol Proced Online     Publication Date:  2009  
Date Detail:
Created Date:  2011-02-01     Completed Date:  2011-07-14     Revised Date:  2011-07-25    
Medline Journal Info:
Nlm Unique ID:  100963717     Medline TA:  Biol Proced Online     Country:  England    
Other Details:
Languages:  eng     Pagination:  161-9     Citation Subset:  -    
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