Document Detail

Comparison of the Delta(12) fatty acid desaturase gene between high-oleic and normal-oleic peanut genotypes.
MedLine Citation:
PMID:  19022202     Owner:  NLM     Status:  MEDLINE    
Delta(12) fatty acid desaturase gene has been targeted as a logical candidate controlling the high oleate trait in peanut seeds. By RT-PCR method, the full-length cDNAs of Delta(12) fatty acid desaturase gene were isolated from peanut (Arachis hypogaea L.) genotypes with normal and high ratio of oleic to linoleic acid, which were designated AhFAD2B and AhFAD2B', respectively. Sequence alignment of their coding regions revealed that an extra A was inserted at the position +442 bp of AhFAD2B' sequence of high oleic acid genotypes, which resulted in the shift of open reading frame and a truncated protein AhFAD2B', with the loss of one histidine box involved in metal ion complex required for the reduction of oxygen. Analysis of transcript level showed that the expression of Delta(12) fatty acid desaturase gene in high oleic acid genotype was slightly lower than that in normal genotype. The enzyme activity experiment of yeast (Saccharomyces cerevisiae) cell transformed with AhFAD2B or AhFAD2B' proved that only AhFAD2B gene product showed significant Delta(12) fatty acid desaturase activity, but AhFAD2B' gene product did not. These results suggested that the change of AhFAD2B' gene sequence resulted in lower activity or deactivation of Delta(12) fatty acid desaturase in high oleic acid genotype.
Shanlin Yu; Lijuan Pan; Qingli Yang; Ping Min; Zengkai Ren; Hongsheng Zhang
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of genetics and genomics = Yi chuan xue bao     Volume:  35     ISSN:  1673-8527     ISO Abbreviation:  J Genet Genomics     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-11-21     Completed Date:  2009-03-30     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  101304616     Medline TA:  J Genet Genomics     Country:  China    
Other Details:
Languages:  eng     Pagination:  679-85     Citation Subset:  IM    
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.
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MeSH Terms
Amino Acid Sequence
Arachis hypogaea / enzymology*,  genetics*,  metabolism
Base Sequence
Cloning, Molecular
DNA, Complementary / genetics
Fatty Acid Desaturases / chemistry,  genetics*,  metabolism
Gene Expression
Gene Expression Regulation, Plant
Molecular Sequence Data
Mutagenesis, Insertional / radiation effects
Oleic Acid / metabolism*
Saccharomyces cerevisiae / cytology,  genetics,  metabolism
Sequence Alignment
Sequence Analysis, DNA
Reg. No./Substance:
0/DNA, Complementary; 112-80-1/Oleic Acid; EC 1.14.19.-/Fatty Acid Desaturases; EC 1.14.99.-/delta-12 fatty acid desaturase

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