| Comparison of the Delta(12) fatty acid desaturase gene between high-oleic and normal-oleic peanut genotypes. | |
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MedLine Citation:
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PMID: 19022202 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Delta(12) fatty acid desaturase gene has been targeted as a logical candidate controlling the high oleate trait in peanut seeds. By RT-PCR method, the full-length cDNAs of Delta(12) fatty acid desaturase gene were isolated from peanut (Arachis hypogaea L.) genotypes with normal and high ratio of oleic to linoleic acid, which were designated AhFAD2B and AhFAD2B', respectively. Sequence alignment of their coding regions revealed that an extra A was inserted at the position +442 bp of AhFAD2B' sequence of high oleic acid genotypes, which resulted in the shift of open reading frame and a truncated protein AhFAD2B', with the loss of one histidine box involved in metal ion complex required for the reduction of oxygen. Analysis of transcript level showed that the expression of Delta(12) fatty acid desaturase gene in high oleic acid genotype was slightly lower than that in normal genotype. The enzyme activity experiment of yeast (Saccharomyces cerevisiae) cell transformed with AhFAD2B or AhFAD2B' proved that only AhFAD2B gene product showed significant Delta(12) fatty acid desaturase activity, but AhFAD2B' gene product did not. These results suggested that the change of AhFAD2B' gene sequence resulted in lower activity or deactivation of Delta(12) fatty acid desaturase in high oleic acid genotype. |
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Authors:
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Shanlin Yu; Lijuan Pan; Qingli Yang; Ping Min; Zengkai Ren; Hongsheng Zhang |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of genetics and genomics = Yi chuan xue bao Volume: 35 ISSN: 1673-8527 ISO Abbreviation: J Genet Genomics Publication Date: 2008 Nov |
Date Detail:
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Created Date: 2008-11-21 Completed Date: 2009-03-30 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 101304616 Medline TA: J Genet Genomics Country: China |
Other Details:
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Languages: eng Pagination: 679-85 Citation Subset: IM |
Affiliation:
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State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Arachis hypogaea / enzymology*, genetics*, metabolism Base Sequence Cloning, Molecular DNA, Complementary / genetics Fatty Acid Desaturases / chemistry, genetics*, metabolism Gene Expression Gene Expression Regulation, Plant Genotype* Molecular Sequence Data Mutagenesis, Insertional / radiation effects Oleic Acid / metabolism* Saccharomyces cerevisiae / cytology, genetics, metabolism Sequence Alignment Sequence Analysis, DNA |
| Chemical | |
Reg. No./Substance:
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0/DNA, Complementary; 112-80-1/Oleic Acid; EC 1.14.19.-/Fatty Acid Desaturases; EC 1.14.99.-/delta-12 fatty acid desaturase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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