| A comparison of dacron versus Flocked nylon swabs for anal cytology specimen collection. | |
| | |
MedLine Citation:
|
PMID: 21791907 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVES: We compared the performance of commonly used Dacron versus flocked nylon swabs for anal cytology. STUDY DESIGN: From 23 HIV-positive men screened at Kaiser Permanente San Francisco (San Francisco, Calif., USA), 2 anal specimens were collected, 1 with each swab in random order, and placed into liquid cytology medium. Specimens were tested for cellularity by quantifying a genomic DNA (erv-3). The number of cells was assessed from prepared slides by automated image analysis. Performance was compared between swabs using 2-sample t tests and standard crossover trial analysis methods accounting for period effect. RESULTS: Flocked swabs collected slightly more erv-3 cells than Dacron for the first sample although not significantly (p = 0.18) and a similar number of erv-3 cells for the second sample (p = 0.85). Flocked swabs collected slightly more cells per slide than the Dacron swabs at both time periods although this was only significant in the second time period (p = 0.42 and 0.03 for first and second periods, respectively). In crossover trial analysis, flocked swabs outperformed Dacron for cell count per slide based on slide imaging (p = 0.03), but Dacron and flocked swabs performed similarly based on erv-3 quantification (p = 0.14). CONCLUSIONS: Further studies should determine whether flocked swabs increase the representation of diagnostically important cells compared to Dacron. |
| | |
Authors:
|
Julia C Gage; Arpita Ghosh; Sylvia Borgonovo; Stephen Follansbee; Nicolas Wentzensen; Patti E Gravitt; Niels Grabe; Bernd Lahrmann; Philip E Castle |
Related Documents
:
|
21684047 - Anticancer activity and cdna microarray studies of a (rs)-1,2,3,5-tetrahydro-4,1-benzox... 22035837 - Beginnings of a good apoptotic meal: the find-me and eat-me signaling pathways. 22171617 - Quantification of plant cell coupling with three-dimensional photoactivation microscopy. 21909927 - Analysis of chemotaxis in dictyostelium. 21043787 - Large-scale manufacture and characterization of a lentiviral vector produced for clinic... 21684047 - Anticancer activity and cdna microarray studies of a (rs)-1,2,3,5-tetrahydro-4,1-benzox... |
Publication Detail:
|
Type: Comparative Study; Journal Article Date: 2011-07-22 |
Journal Detail:
|
Title: Acta cytologica Volume: 55 ISSN: 0001-5547 ISO Abbreviation: Acta Cytol. Publication Date: 2011 |
Date Detail:
|
Created Date: 2011-07-27 Completed Date: 2011-09-20 Revised Date: 2013-05-23 |
Medline Journal Info:
|
Nlm Unique ID: 0370307 Medline TA: Acta Cytol Country: Switzerland |
Other Details:
|
Languages: eng Pagination: 364-7 Citation Subset: IM |
Copyright Information:
|
Copyright © 2011 S. Karger AG, Basel. |
Affiliation:
|
Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, DHHS, Bethesda, MD 20852, USA. gagej@mail.nih.gov |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Anal Canal
/
pathology*,
virology Anus Neoplasms / pathology*, virology Cytodiagnosis DNA, Viral / analysis HIV Seropositivity / pathology Humans Male Nylons* Papillomaviridae / genetics, isolation & purification* Papillomavirus Infections / pathology*, virology Polyethylene Terephthalates* Prognosis Specimen Handling / instrumentation*, methods |
| Chemical | |
Reg. No./Substance:
|
0/DNA, Viral; 0/Nylons; 0/Polyethylene Terephthalates |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Simultaneous Detection of T Lymphocyte-Related Antigens (CD4/CD8, CD57, TCR?) with Nuclei by Fluores...
Next Document: Uterine cervical sarcoidosis identified on pap test: cytomorphologic findings and differential diagn...