| Comparison of commercial ELISA blood tests for early pregnancy detection in dairy cows. | |
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MedLine Citation:
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PMID: 20881353 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The objective of the present study was to compare two commercially available blood-based pregnancy tests, namely BioPRYN, an ELISA for pregnancy-specific protein B (PSPB), and an ELISA for pregnancy-associated glycoprotein (PAG), for early pregnancy diagnosis in dairy cattle using transrectal ultrasonography as a gold standard. Transrectal ultrasonography was conducted 26-58 days after artificial insemination (AI) in 197 cattle from 19 farms. Concurrently, a blood sample was collected for determination of serum PSPB and PAG. Transrectal palpation was performed approximately 120 days after AI to verify that pregnancy was maintained. For PSPB and PAG, there were no significant differences (P>0.05) in sensitivity (98.0 and 97.8%), specificity (97.1 and 91.2%), positive predictive values (99.3 and 97.8%), negative predictive values (91.9 and 91.2%) and accuracy (97.8 and 96.4%). In conclusion, the two blood pregnancy assays were equally efficacious and were highly accurate (based on transrectal ultrasonography as the gold standard). |
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Authors:
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Marion Piechotta; Julia Bollwein; Morten Friedrich; Thomas Heilkenbrinker; Chuck Passavant; Josh Branen; Garth Sasser; Martina Hoedemaker; Heinrich Bollwein |
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Publication Detail:
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Type: Comparative Study; Journal Article Date: 2010-09-24 |
Journal Detail:
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Title: The Journal of reproduction and development Volume: 57 ISSN: 1348-4400 ISO Abbreviation: J. Reprod. Dev. Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-03-22 Completed Date: 2011-08-04 Revised Date: 2011-12-13 |
Medline Journal Info:
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Nlm Unique ID: 9438792 Medline TA: J Reprod Dev Country: Japan |
Other Details:
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Languages: eng Pagination: 72-5 Citation Subset: IM |
Affiliation:
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Clinic for Cattle, University of Veterinary Medicine Hannover, Hannover, Germany. marion.piechotta@tiho-hannover.de |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Breeding Cattle Dairying / methods* Enzyme-Linked Immunosorbent Assay / veterinary Female Glycoproteins / blood Insemination, Artificial / veterinary Pregnancy Pregnancy Proteins / blood Pregnancy Tests, Immunologic / veterinary* Pregnancy, Animal / blood* Reagent Kits, Diagnostic / veterinary* Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/Glycoproteins; 0/Pregnancy Proteins; 0/Reagent Kits, Diagnostic |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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