| Comparison of Cobe Spectra and Haemonetics MCS-3P cell separators for peripheral blood stem cell harvesting. | |
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MedLine Citation:
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PMID: 8547869 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A prospective study was undertaken to compare the total nucleated cell (TNC), mononuclear cell (MNC), CD34+ cell, and CFU-GM yields of two different cell separators. A Haemonetics MCS-3P and a Cobe Spectra machine were used to leukapherese 10 patients with malignant diseases on 4 consecutive days after mobilization with G-CSF. All patients were harvested twice on each machine for a fixed period of time. The blood volume processed (10.47 vs 3.79 l, P < 0.001), MNC yield (2.66 vs 0.90 x 10(8)/kg; P < 0.001), MNC yield rate (1.66 vs 0.55 x 10(6)/kg/min; P < 0.001), MNC purity (81 vs 42%; P < 0.001), CFU-GM yield (18.1 vs 5.5 x 10(4)/kg; P = 0.001), and CFU-GM yield rate (11.27 vs 3.42 x 10(2)/kg/min; P = 0.001) were significantly higher with the Spectra. The CD34+ cell yields and yield rates were comparable. Although CFU-GM and MNC yields per unit blood volume processed were comparable for both machines, there was a trend for higher CD34+ yields per unit volume processed with MCS-3P. We conclude that Spectra is faster than MCS-3P with more blood processed per unit time resulting in higher cell yields, but yields per unit volume processed are comparable for both machines. The choice of machine for a given patient depends upon convenience, venous access and the time available. |
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Authors:
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J Mehta; R Powles; S Cabral; V Shepherd; S Singhal; R Morilla; J Treleaven |
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Publication Detail:
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Type: Clinical Trial; Comparative Study; Controlled Clinical Trial; Journal Article |
Journal Detail:
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Title: Bone marrow transplantation Volume: 16 ISSN: 0268-3369 ISO Abbreviation: Bone Marrow Transplant. Publication Date: 1995 Nov |
Date Detail:
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Created Date: 1996-02-20 Completed Date: 1996-02-20 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8702459 Medline TA: Bone Marrow Transplant Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 707-9 Citation Subset: IM |
Affiliation:
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Leukaemia Unit, Royal Marsden Hospital, Sutton, Surrey, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Antigens, CD34 / analysis Hematopoietic Stem Cell Transplantation* Humans Leukapheresis / instrumentation* Middle Aged Prospective Studies |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD34 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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