Document Detail


Comparison of chromogenic Biolog Rainbow agar Shigella/Aeromonas with xylose lysine desoxycholate agar for isolation and detection of Shigella spp. from foods.
MedLine Citation:
PMID:  20819355     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Shigella outbreaks are widely reported throughout the world. However, it remains a challenge to isolate Shigella spp. from foods by using conventional microbiological media. The main objective of this study was to determine the effectiveness of a novel chromogenic medium, Rainbow agar Shigella/Aeromonas (Rainbow agar), for the isolation and detection of Shigella spp. in foods. All four Shigella species, S. sonnei, S. flexneri, S. dysenteriae, and S. boydii, were studied. Rainbow agar was compared with tryptic soy agar, xylose lysine desoxycholate agar (XLD), and Salmonella Shigella agar (SSA) for enumeration of Shigella spp. in pure culture. This chromogenic agar and XLD were also used to isolate Shigella spp. in artificially contaminated foods (4.8 log CFU/g of food), including lettuce, parsley, cilantro, spinach, potato salad, and shrimp. The inhibitory effect on Shigella growth by Rainbow agar was between that of XLD and SSA. All vegetables studied showed a moderately high background microflora on XLD and Rainbow agar. With artificially inoculated produce, Rainbow agar recovered about 1 to 2 log CFU more S. sonnei, S. dysenteriae, and S. boydii per g of food than did XLD. For potato salad and shrimp, which had low background microflora on Rainbow agar, Rainbow agar was slightly better in recovering Shigella spp. than XLD was in most cases. However, we found that the addition of streptomycin (6.25 mg/liter) to Rainbow agar could facilitate the isolation of Shigella in vegetables tested. In conclusion, Rainbow agar was a much more effective medium than was XLD for the isolation of Shigella spp. from foods.
Authors:
Guodong Zhang; Keith A Lampel
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of food protection     Volume:  73     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-09-07     Completed Date:  2010-10-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1458-65     Citation Subset:  IM    
Affiliation:
Center for Food Safety and Applied Nutrition, HFS-712, U.S. Food and Drug Administration, 5100 Paint Branch Parkway, College Park, Maryland 20740, USA.
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MeSH Terms
Descriptor/Qualifier:
Aeromonas / classification,  growth & development,  isolation & purification*
Agar / chemistry*
Bacteriological Techniques*
Chromogenic Compounds / metabolism
Colony Count, Microbial
Consumer Product Safety
Culture Media / chemistry
Deoxycholic Acid / metabolism
Food Contamination / analysis*
Humans
Lysine / metabolism
Shigella / classification,  growth & development,  isolation & purification*
Xylose / metabolism
Chemical
Reg. No./Substance:
0/Chromogenic Compounds; 0/Culture Media; 0/Xylose; 56-87-1/Lysine; 83-44-3/Deoxycholic Acid; 9002-18-0/Agar

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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