Document Detail


Comparison of Chlamydia trachomatis serovar L2 growth in polarized genital epithelial cells grown in three-dimensional culture with non-polarized cells.
MedLine Citation:
PMID:  18396437     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A common model for studying Chlamydia trachomatis and growing chlamydial stocks uses Lymphogranuloma venereum serovar L2 and non-polarized HeLa cells. However, recent publications indicate that the growth rate and progeny yields can vary considerably for a particular strain depending on the cell line/type used, and seem to be partially related to cell tropism. In the present study, the growth of invasive serovar L2 was compared in endometrial HEC-1B and endocervical HeLa cells polarized on collagen-coated microcarrier beads, as well as in HeLa cells grown in tissue culture flasks. Microscopy analysis revealed no difference in chlamydial attachment/entry patterns or in inclusion development throughout the developmental cycle between cell lines. Very comparable growth curves in both cell lines were also found using real-time PCR analysis, with increases in chlamydial DNA content of 400-500-fold between 2 and 36 h post-inoculation. Similar progeny yields with comparable infectivity were recovered from HEC-1B and HeLa cell bead cultures, and no difference in chlamydial growth was found in polarized vs. non-polarized HeLa cells. In conclusion, unlike other C. trachomatis strains such as urogenital serovar E, invasive serovar L2 grows equally well in physiologically different endometrial and endocervical environments, regardless of the host cell polarization state.
Authors:
Sophie Dessus-Babus; Cheryl G Moore; Judy D Whittimore; Priscilla B Wyrick
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural     Date:  2008-02-21
Journal Detail:
Title:  Microbes and infection / Institut Pasteur     Volume:  10     ISSN:  1286-4579     ISO Abbreviation:  Microbes Infect.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-05-26     Completed Date:  2008-07-15     Revised Date:  2014-09-10    
Medline Journal Info:
Nlm Unique ID:  100883508     Medline TA:  Microbes Infect     Country:  France    
Other Details:
Languages:  eng     Pagination:  563-70     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Chlamydia trachomatis / growth & development*,  isolation & purification
DNA, Bacterial / analysis
Endometrium / microbiology*,  ultrastructure
Female
HeLa Cells
Humans
Microscopy, Electron, Transmission
Microscopy, Fluorescence
Microspheres
Polymerase Chain Reaction
Grant Support
ID/Acronym/Agency:
AI13446/AI/NIAID NIH HHS; R01 AI013446/AI/NIAID NIH HHS; R01 AI013446-29/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Bacterial
Comments/Corrections

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