| Comparison of candidate materials for a synthetic osteo-odonto keratoprosthesis device. | |
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MedLine Citation:
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PMID: 20739467 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: Osteo-odonto keratoprosthesis is one of the most successful forms of keratoprosthesis surgery for end-stage corneal and ocular surface disease. There is a lack of detailed comparison studies on the biocompatibilities of different materials used in keratoprosthesis. The aim of this investigation was to compare synthetic bioinert materials used for keratoprosthesis surgery with hydroxyapatite (HA) as a reference. METHODS: Test materials were sintered titanium oxide (TiO(2)), aluminum oxide (Al(2)O(3)), and yttria-stabilized zirconia (YSZ) with density >95%. Bacterial adhesion on the substrates was evaluated using scanning electron microscopy and the spread plate method. Surface properties of the implant discs were scanned using optical microscopy. Human keratocyte attachment and proliferation rates were assessed by cell counting and MTT assay at different time points. Morphologic analysis and immunoblotting were used to evaluate focal adhesion formation, whereas cell adhesion force was measured with a multimode atomic force microscope. RESULTS: The authors found that bacterial adhesion on the TiO(2), Al(2)O(3), and YSZ surfaces were lower than that on HA substrates. TiO(2) significantly promoted keratocyte proliferation and viability compared with HA, Al(2)O(3,) and YSZ. Immunofluorescent imaging analyses, immunoblotting, and atomic force microscope measurement revealed that TiO(2) surfaces enhanced cell spreading and cell adhesion compared with HA and Al(2)O(3). CONCLUSIONS: TiO(2) is the most suitable replacement candidate for use as skirt material because it enhanced cell functions and reduced bacterial adhesion. This would, in turn, enhance tissue integration and reduce device failure rates during keratoprosthesis surgery. |
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Authors:
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Xiao Wei Tan; A Promoda P Perera; Anna Tan; Donald Tan; K A Khor; Roger W Beuerman; Jodhbir S Mehta |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2011-01-05 |
Journal Detail:
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Title: Investigative ophthalmology & visual science Volume: 52 ISSN: 1552-5783 ISO Abbreviation: Invest. Ophthalmol. Vis. Sci. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-01-06 Completed Date: 2011-02-07 Revised Date: 2011-03-18 |
Medline Journal Info:
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Nlm Unique ID: 7703701 Medline TA: Invest Ophthalmol Vis Sci Country: United States |
Other Details:
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Languages: eng Pagination: 21-9 Citation Subset: IM |
Affiliation:
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Singapore Eye Research Institute, Singapore. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aluminum Oxide
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metabolism Alveolar Process / transplantation Bacterial Adhesion / physiology* Biocompatible Materials / metabolism* Blotting, Western Cell Adhesion / physiology* Cell Proliferation Cell Survival Cells, Cultured Corneal Diseases / surgery Corneal Stroma / cytology Cuspid / transplantation Cytoskeletal Proteins / metabolism Durapatite / metabolism Fibroblasts / metabolism* Humans Microscopy, Atomic Force Microscopy, Electron, Scanning Prostheses and Implants* Staphylococcus aureus / physiology, ultrastructure Titanium / metabolism Yttrium / metabolism Zirconium / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Biocompatible Materials; 0/Cytoskeletal Proteins; 0/yttria stabilized tetragonal zirconia; 1306-06-5/Durapatite; 1344-28-1/Aluminum Oxide; 13463-67-7/titanium dioxide; 7440-32-6/Titanium; 7440-65-5/Yttrium; 7440-67-7/Zirconium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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