Document Detail


Comparison between 5-aminosalicylic acid (5-ASA) and para-aminosalicylic acid (4-PAS) as potential protectors against Mn-induced neurotoxicity.
MedLine Citation:
PMID:  23315311     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Manganese (Mn) is an essential metal for biological systems; however, occupational or clinical exposure to high levels of Mn can produce a neurological disorder called manganism. Oxidative stress and neuroinflammation play major roles in the Mn-induced neurodegeneration leading to dysfunction of the basal ganglia. We investigated the toxic effects of MnCl2 in an immortalized rat brain endothelial cell line (RBE4) and the protective effects of the radical scavenging aminosalicylic acids, 5-aminosalicylic acid (5-ASA) and 4-aminosalicylic acid (4-PAS). Mn cytotoxicity was determined with 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) reduction and lactate dehydrogenase (LDH) activity. A significant decrease in MTT reduction concomitant with increased LDH release was noted in RBE4 cells exposed for 24 h to MnCl2 (600 and 800 μM; p < 0.0001). Our results establish that compared to 4-PAS, 5-ASA has greater efficacy in protecting RBE4 cells from Mn-induced neurotoxicity after preexposure to MnCl2 800 μM (p < 0.0001).
Authors:
Dinamene Santos; M Camila Batoreu; Michael Aschner; Ana P Marreilha dos Santos
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-01-15
Journal Detail:
Title:  Biological trace element research     Volume:  152     ISSN:  1559-0720     ISO Abbreviation:  Biol Trace Elem Res     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-03-08     Completed Date:  2013-08-29     Revised Date:  2014-04-02    
Medline Journal Info:
Nlm Unique ID:  7911509     Medline TA:  Biol Trace Elem Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  113-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Aminosalicylic Acid / pharmacology*
Analysis of Variance
Animals
Brain / cytology
Cell Line
Cell Survival / drug effects
Chlorides / toxicity
Dose-Response Relationship, Drug
Endothelial Cells / cytology,  drug effects,  metabolism
Humans
L-Lactate Dehydrogenase / metabolism
Manganese / toxicity*
Manganese Compounds
Manganese Poisoning / etiology,  prevention & control*
Mesalamine / pharmacology*
Neuroprotective Agents / pharmacology
Rats
Grant Support
ID/Acronym/Agency:
P30 ES000267/ES/NIEHS NIH HHS; R01 ES 10563/ES/NIEHS NIH HHS; R01 ES010563/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Chlorides; 0/Manganese Compounds; 0/Neuroprotective Agents; 42Z2K6ZL8P/Manganese; 4Q81I59GXC/Mesalamine; 5B2658E0N2/Aminosalicylic Acid; EC 1.1.1.27/L-Lactate Dehydrogenase; QQE170PANO/manganese chloride
Comments/Corrections

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