| Comparison of autoanalyzer and back titration for measurement of bicarbonate concentration in endoscopically collected pancreatic fluid. | |
| | |
MedLine Citation:
|
PMID: 20966809 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
OBJECTIVES: : In secretin-stimulated pancreatic function testing, the standard technique for bicarbonate measurement is back titration (BT). Chemistry autoanalyzers (AAs) automate bicarbonate measurement and are universally available; however, this method has not been validated in pancreatic fluid. The aims of the study were (1) to compare the AA and BT for measurement of bicarbonate in pancreatic fluid and (2) to determine the effects of variable conditions on bicarbonate concentration. METHODS: : Pancreatic fluid samples were analyzed for bicarbonate concentration using both BT and AA. Additional experiments were conducted to determine the effect of different sample conditions. RESULTS: : The results obtained by BT and AA were highly concordant (Lin concordance coefficient, 0.96). An AA cutpoint of 84 mM optimized sensitivity (91.1%) and specificity (94.1%) compared with the BT reference standard. Blood contamination spuriously increased the bicarbonate, an effect that was eliminated by centrifugation. The bicarbonate levels were not significantly affected by delayed analysis, storage condition, nitrogen gas treatment, or the addition of mineral oil or protease inhibitors. CONCLUSIONS: : The bicarbonate concentrations obtained by AA are highly concordant with those obtained by BT. Secretin pancreatic function testing fluid analysis may now be performed in any hospital. |
| | |
Authors:
|
Zuoliang Xiao; Rocio Lopez; Mansour A Parsi; Milan Dodig; Tyler Stevens |
Related Documents
:
|
8126159 - The iodine perchlorate discharge test before and after one year of methimazole treatmen... 6332529 - A single-specimen fecal chymotrypsin test in the diagnosis of pancreatic insufficiency:... 3612029 - Isoamylase measurements with monoclonal antibody test strips. 9187069 - Rapid measurement of urinary trypsinogen-2 as a screening test for acute pancreatitis. 3802469 - Total or free thyroxin as a primary test of thyroid function. 17640179 - Absence of cardiovascular autonomic dysfunction and vagal pancreatic impairment in idio... 21914549 - Comparison of a rapid diagnostic test and microimmunofluorescence assay for detecting a... 6414729 - On the toxic effects of tetraethyl lead and its derivatives on the chrysophyte poterioo... 20059199 - Permutations and time series analysis. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Pancreas Volume: 40 ISSN: 1536-4828 ISO Abbreviation: Pancreas Publication Date: 2011 Mar |
Date Detail:
|
Created Date: 2011-02-11 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8608542 Medline TA: Pancreas Country: United States |
Other Details:
|
Languages: eng Pagination: 237-41 Citation Subset: IM |
Affiliation:
|
From the *Digestive Disease Institute and †Department of Quantitative Health Sciences, Cleveland Clinic, Cleveland, OH. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Reinforcement of pancreticojejunostomy using polyglycolic acid mesh and fibrin glue sealant.
Next Document: Superoxide dismutase and nicotinamide adenine dinucleotide phosphate: quinone oxidoreductase polymor...