| Comparison of 1,2-propanediol and ethylene glycol for cryopreservation of slow-cooled mouse zygotes and their subsequent development. | |
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MedLine Citation:
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PMID: 12877255 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: This study was conducted on cryoprotective activity of ethylene glycol (ETG) and propanediol (PROH) on cleavage rate of mouse zygotes. METHODS: Mouse oocytes were excised from fallopian tube of gonadotropin-treated mice, then inseminated with spermatozoa. After 16.5-17.5 h, zygotes were randomly allocated into three groups; control, toxicity, and frozen. In the latter, zygotes were slowly cooled with ETG and PROH similar to those used for human embryo cryopreservation. The survived zygotes cultured for 120 h and their later stages of development were compared with nonfrozen embryos. RESULTS: The toxicity test showed that no differences were observed in cleavage rate between exposed and nonexposed embryos. The survival and expanded hatching blastocyst rate of embryos frozen with PROH was significantly better than with ETG (92.8 vs. 58.2% and 68.2 vs. 39.1%, respectively). CONCLUSIONS: ETG does not appear to be a good alternative to the classical PROH for freezing of mouse zygotes. |
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Authors:
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Hassan Hassani Bafrani; Naser Salsabil; Parichehr Pasbakhsh; Hossein Hassani; Mansoureh Movahedin; Taghi Al-tarihi; Firoozeh Akbari; Mansour Keshavarz |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of assisted reproduction and genetics Volume: 20 ISSN: 1058-0468 ISO Abbreviation: J. Assist. Reprod. Genet. Publication Date: 2003 Jun |
Date Detail:
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Created Date: 2003-07-24 Completed Date: 2004-03-30 Revised Date: 2013-03-11 |
Medline Journal Info:
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Nlm Unique ID: 9206495 Medline TA: J Assist Reprod Genet Country: United States |
Other Details:
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Languages: eng Pagination: 234-40 Citation Subset: IM |
Affiliation:
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Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. hasanhasani@yahoo.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cryopreservation / methods* Cryoprotective Agents / pharmacology* Ethylene Glycol / pharmacology* Female Fertilization in Vitro Male Mice Propylene Glycol / pharmacology* Spermatozoa / cytology, physiology Zygote / cytology*, physiology* |
| Chemical | |
Reg. No./Substance:
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0/Cryoprotective Agents; 107-21-1/Ethylene Glycol; 57-55-6/Propylene Glycol |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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