Document Detail

Comparision between sonodynamic effects with protoporphyrin IX and hematoporphyrin on the cytoskeleton of Ehrlich ascites carcinoma cells.
MedLine Citation:
PMID:  20187797     Owner:  NLM     Status:  MEDLINE    
PURPOSE: Our previous work demonstrated that the enhancement of cytotoxicity by protoporphyrin IX (Pp) was significantly higher than hematoporphyrin (Hp), when they were compared at the same ultrasound-exposure conditions, since the cytoskeleton plays a crucial role in numerous cell functions and the effect of sonodynamic therapy (SDT) on cytoskeleton has not been reported yet. So, it is very important to investigate whether SDT has some influence on the cytoskeleton, and it would also be interesting to compare Pp-/ and Hp-mediated SDT on the cytoskeleton. METHODS: Ehrlich ascites carcinoma (EAC) cells were exposed to ultrasound for up to 15 seconds (1.34 MHz, 1 w/cm(2)) in the presence of 20 microM of Pp or 20 microM of Hp. Cell viability was determined by the trypan blue exclusion test. Morphologic changes were observed under a scanning electron microscope. Cytoskeleton damages were shown by immunofluorescence staining of microtubules, actin microfilaments, and intermediate filaments of vimentin. RESULTS: Our experiment showed that the extent of cytoskeleton damage in EAC cells increased as time was prolonged, and also which was more significant in the Pp-SDT group than that in the Hp-SDT group at the same time after exposure. CONCLUSIONS: Results implied that the ultrastructural changes might account for cell destruction and the cytoskeletal components were involved in the cell-damaging process induced SDT treatment.
Pan Wang; Xiaobing Wang; Quanhong Liu; Xia Zhao; Bing Cao; Ping Zhao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer biotherapy & radiopharmaceuticals     Volume:  25     ISSN:  1557-8852     ISO Abbreviation:  Cancer Biother. Radiopharm.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-03-01     Completed Date:  2010-10-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9605408     Medline TA:  Cancer Biother Radiopharm     Country:  United States    
Other Details:
Languages:  eng     Pagination:  55-64     Citation Subset:  IM    
College of Life Sciences, Shaanxi Normal University, Xi'an, China.
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MeSH Terms
Actins / metabolism
Carcinoma, Ehrlich Tumor / metabolism,  pathology,  therapy*
Cell Death / drug effects
Cell Line, Tumor
Cytoskeleton / drug effects*,  metabolism,  pathology
Fluorescent Antibody Technique, Indirect
Hematoporphyrins / chemistry,  pharmacology*
Mice, Inbred ICR
Photosensitizing Agents / chemistry,  pharmacology*
Protoporphyrins / chemistry,  pharmacology*
Ultrasonic Therapy / methods*
Vimentin / metabolism
Reg. No./Substance:
0/Actins; 0/Hematoporphyrins; 0/Photosensitizing Agents; 0/Protoporphyrins; 0/Vimentin; 553-12-8/protoporphyrin IX

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