| Comparing electron microscopy and a competitive blocking ELISA in the detection of rotaviruses in porcine faeces. | |
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MedLine Citation:
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PMID: 20036588 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Analysis of 476 faecal samples from diarrhoeic piglets was performed using electron microscopy (EM) and a competitive blocking (CB)-ELISA based on monoclonal antibodies to the VP6 protein of group A rotavirus. Rotavirus was detected by EM and/or CB-ELISA in 111 (23.3%) samples. Of these, all groups of rotavirus were identified in 83 (74.8%) samples by EM (EM+), while group A rotavirus was identified in 90 (81.1%) samples by CB-ELISA (ELISA+). However, only 62 (55.9%) of samples were positive using both detection methods. The finding of 28 (25.2%) EM-/CB-ELISA+ samples illustrated the high sensitivity of the CB-ELISA method. On PCR analysis, groups B and C rotavirus was found in 3 and 16 of 19 EM+/CB-ELISA- samples, respectively. Although the study illustrates the high sensitivity of a CB-ELISA in rotavirus detection, the findings highlight the need to use a range of diagnostic methods in detecting these viruses in clinical samples. |
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Authors:
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Ladislav Rodák; Radka Smítalová; Bedřich Smíd; Zora Nevoránková; Lubomír Valíček; Ivan Pšikal |
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Publication Detail:
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Type: Journal Article Date: 2009-12-29 |
Journal Detail:
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Title: Veterinary journal (London, England : 1997) Volume: 187 ISSN: 1532-2971 ISO Abbreviation: Vet. J. Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-02-18 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9706281 Medline TA: Vet J Country: England |
Other Details:
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Languages: eng Pagination: 279-81 Citation Subset: IM |
Copyright Information:
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Copyright © 2009 Elsevier Ltd. All rights reserved. |
Affiliation:
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Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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