Document Detail


Comparative venom toxicity between Pteromalus puparum and Nasonia vitripennis (Hymenoptera: Pteromalidae) toward the hemocytes of their natural hosts, non-target insects and cultured insect cells.
MedLine Citation:
PMID:  16026808     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Crude venoms from two parasitoid species, Pteromalus puparum and Nasonia vitripennis (Hymenoptera: Pteromalidae) were assayed for biological activities toward hemocytes from two species of their natural hosts and eight species of their non-natural hosts as well as two lines of cultured Lepidoptera cells, respectively. By inhibiting the spreading and viability of insect hemocytes, the venom from P. puparum displayed significantly higher activities toward plasmatocytes and granular cells from both larvae and pupae of two natural hosts, Pieris rapae and Papilio xuthus, and lower activity toward those from Spodoptera litura, Musca domestica and Sarcophaga peregrina. However, no effect was found towards any type of hemocytes from other five insects tested, namely, Ectropis oblique, Galleria mellonella, Sesamia inferens, Bombyx mori and Parnara guttata. In contrast, the venom from N. vitripennis showed a narrower range of targeted insects. It appeared to have highly adverse effects on the spreading and viability of plasmatocytes and granular cells only from the natural hosts, M. domestica and S. peregrina, little toxicity to cells from P. rapae and P. xuthus, and no effect on any of the other insects tested. Pteromalus puparum venom also apparently presented a high ability to block the spreading of Tn-5B1-4 cells derived from Trichoplusia ni, and high cytotoxicity to the cells and Ha cells derived from Helicoverpa armigera. Nasonia vitripennis venom, however, only had a marked lethal effect to Ha cells. In addition, the possibility that the host range of a defined parasitoid could be assessed using our method of treating hemocytes from candidate insects with venom in vitro, and the potential of our venoms tested in the development of bio-insecticides, insect-resistant transgenic plants, are discussed.
Authors:
Zhong Zhang; Gong-Yin Ye; Jun Cai; Cui Hu
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Toxicon : official journal of the International Society on Toxinology     Volume:  46     ISSN:  0041-0101     ISO Abbreviation:  Toxicon     Publication Date:  2005 Sep 
Date Detail:
Created Date:  2005-08-01     Completed Date:  2005-10-19     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  1307333     Medline TA:  Toxicon     Country:  England    
Other Details:
Languages:  eng     Pagination:  337-49     Citation Subset:  IM    
Affiliation:
State Key Laboratory of Rice Biology, Institute of Applied Entomology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Granulocytes / cytology,  drug effects
Hemocytes / cytology,  drug effects*
Host-Parasite Interactions
Hymenoptera / chemistry*
Lepidoptera / cytology,  drug effects
Species Specificity
Time Factors
Venoms / toxicity*
Wasps / chemistry
Chemical
Reg. No./Substance:
0/Venoms

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