Document Detail

Comparative ultrastructure and cytochemistry of epidermal responses to tape stripping, ethanol and vitamin A acid in hairless mice.
MedLine Citation:
PMID:  224115     Owner:  NLM     Status:  MEDLINE    
The responses of hairless mouse epidermis to tape stripping, ethanol and vitamin A acid were compared using electron microscopy and cytochemistry. Stripping the skin 4 times with cellophane tape removed most of the stratum corneum and caused the development of enlarged intercellular spaces and dense intramitochondrial inclusions. These changes began within an hour, reached a maximum by 24 hr, and subsided by about 4 days. There was also some accumulation of glycogen and the development of occasional basal lamina breaks and a few intracellular lipid droplets by 24 hr. Topically applied ethanol (95%) produced a similar response, though less damaging. In addition, ethanol induced the formation of numerous lipid droplets in the cytoplasm of keratinocytes by 24 hr and often caused sloughing of the stratum corneum. Vitamin A acid (3%) was dissolved in propylene glycol for application to the skin. We found the application of propylene glycol alone to produce no epidermal changes. Vitamin A acid produced mild damaging effects with small intercellular spaces and intramitochondrial inclusions, but no lipid droplets or glycogen were detected. Vitamin A acid also caused dramatic inhibition of keratinization by 24 hr. Intramitochondrial inclusions were digested by protease, indicating a protein component and they also stained with silver methenamine, indicating the presence of glycogen or glycoprotein.
W R Brown; J E Habowsky
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  The Journal of investigative dermatology     Volume:  73     ISSN:  0022-202X     ISO Abbreviation:  J. Invest. Dermatol.     Publication Date:  1979 Sep 
Date Detail:
Created Date:  1979-10-26     Completed Date:  1979-10-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0426720     Medline TA:  J Invest Dermatol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  203-6     Citation Subset:  IM    
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MeSH Terms
Ethanol / pharmacology*
Glycogen / analysis
Inclusion Bodies / ultrastructure
Lipids / analysis
Mice, Nude
Microscopy, Electron
Skin / drug effects*,  metabolism,  ultrastructure
Tretinoin / pharmacology*
Vitamin A / analogs & derivatives*
Reg. No./Substance:
0/Lipids; 11103-57-4/Vitamin A; 302-79-4/Tretinoin; 64-17-5/Ethanol; 9005-79-2/Glycogen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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