Document Detail

Comparative toxicity of oleic and linoleic acid on human lymphocytes.
MedLine Citation:
PMID:  16236329     Owner:  NLM     Status:  MEDLINE    
Commercially available lipid emulsions for parenteral nutrition are mainly composed by long chain triacylglycerol containing a high proportion of linoleic acid (LA) or oleic acid (OA). The immunological impact of such therapy is particularly important because parenteral diets are often administered to critically ill patients as a mechanism to supply adequate nutrition during catabolic stress conditions. The comparative toxicity of OA and LA on human lymphocytes and the type of cell death induced by these fatty acids were determined in vitro. Parameters of cell death were investigated by flow cytometry-cell viability, DNA fragmentation, phosphatidylserine externalization, mitochondrial depolarization, neutral lipid accumulation and production of reactive oxygen species-and by fluorescence microscopy-chromatin condensation. Additionally a spectrofluorometric assay was employed to determine the activities of caspase--3, 6 and 8. Evidence is presented herein that OA is less toxic to human lymphocytes than LA. However, both fatty acids promoted apoptosis and necrosis of these cells. The mechanism of cell death induced by OA involved activation of caspase 3 while the mechanism of death induced by LA involved mitochondrial depolarization and ROS production. Importantly, neutral lipid accumulation may be a mechanism to protect lymphocytes against the toxicity induced by OA. OA may offer an immunological less problematic alternative to LA with respect to fatty acid composition of parenteral nutritional emulsions.
Maria F Cury-Boaventura; Renata Gorjão; Thaís Martins de Lima; Philip Newsholme; Rui Curi
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-10-19
Journal Detail:
Title:  Life sciences     Volume:  78     ISSN:  0024-3205     ISO Abbreviation:  Life Sci.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-02-15     Completed Date:  2006-04-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  1448-56     Citation Subset:  IM    
Department of Physiology and Biophysics, Institute of Biomedical Sciences, Av. Prof. Lineu Prestes, 1524, CEP 05508-900, University of São Paulo, SP, Brazil.
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MeSH Terms
Cell Survival / drug effects
Cells, Cultured
Linoleic Acid / toxicity*
Lymphocytes / drug effects,  pathology*
Membrane Potentials / drug effects,  physiology
Mitochondria / physiology
Monocytes / cytology,  drug effects
Oleic Acid / toxicity*
Phosphatidylserines / blood
Reactive Oxygen Species / metabolism
Reg. No./Substance:
0/Phosphatidylserines; 0/Reactive Oxygen Species; 112-80-1/Oleic Acid; 2197-37-7/Linoleic Acid

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