Document Detail


Comparative study of the use of neuroblastoma cells (Neuro-2a) and neuroblastomaxglioma hybrid cells (NG108-15) for the toxic effect quantification of marine toxins.
MedLine Citation:
PMID:  18657563     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The suitability and sensitivity of two neural cell models, NG108-15 and Neuro-2a, to different marine toxins were evaluated under different incubation and exposure times and in the presence or absence of ouabain and veratridine (O/V). NG108-15 cells were more sensitive to pectenotoxin-2 than Neuro-2a cells. For saxitoxin, brevetoxin-3, palytoxin, okadaic acid and dinophysistoxin-1 both cell types proved to be sensitive and suitable for toxicity evaluation. For domoic acid preliminary results were presented. Setting incubation time and exposure time proved to be critical for the development of the assays. In order to reduce the duration of the assays, it was better to reduce cell time incubation previous to toxin exposure than exposure time. For palytoxin, after 24h of growth, both cell types were sensitive in the absence of O/V. When growth time previous to toxin exposure was reduced, both cell types were unsensitive to palytoxin when O/V was absent. Although dinophysistoxin-1 and okadaic acid are both phosphatase inhibitors, these toxins did not respond similarly in front of the experimental conditions studied. Both cell types were able to identify Na-channel acting toxins and allowed to quantify the effect of saxitoxin, brevetoxin-3, palytoxin, okadaic acid, dinophysistoxin-1 and pectenotoxin-2 under different experimental conditions.
Authors:
Elisabeth Cañete; Jorge Diogène
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2008-07-09
Journal Detail:
Title:  Toxicon : official journal of the International Society on Toxinology     Volume:  52     ISSN:  0041-0101     ISO Abbreviation:  Toxicon     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-09-12     Completed Date:  2008-11-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1307333     Medline TA:  Toxicon     Country:  England    
Other Details:
Languages:  eng     Pagination:  541-50     Citation Subset:  IM    
Affiliation:
Centre d' Aqüicultura, IRTA, Ctra Poble Nou s/n Km 5.5, 43540 Sant Carles de la Rapita, Tarragona, Spain.
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MeSH Terms
Descriptor/Qualifier:
Acrylamides / toxicity
Animals
Cell Line, Tumor*
Dose-Response Relationship, Drug
Furans / toxicity
Glioma
Hybrid Cells
Kainic Acid / analogs & derivatives,  toxicity
Marine Toxins / toxicity*
Mice
Neuroblastoma
Okadaic Acid / toxicity
Oxocins / toxicity
Pyrans / toxicity
Saxitoxin / toxicity
Time Factors
Toxicity Tests / methods*
Chemical
Reg. No./Substance:
0/Acrylamides; 0/Furans; 0/Marine Toxins; 0/Oxocins; 0/Pyrans; 0/brevetoxin 3, Karenia brevis; 11077-03-5/palytoxin; 14277-97-5/domoic acid; 35523-89-8/Saxitoxin; 487-79-6/Kainic Acid; 78111-17-8/Okadaic Acid; 81720-10-7/dinophysistoxin 1; 97564-91-5/pectenotoxin 2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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