Document Detail


Comparative study of the mutagenic and genotoxic activity associated with inhalable particulate matter in Rio de Janeiro air.
MedLine Citation:
PMID:  2137085     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have determined the genotoxic and mutagenic activities associated with inhalable particulate matter (IPM) collected in Rio de Janeiro, Brazil, Camden, NJ, and Caldecott Tunnel, CA, and used these results to compare three different bioassays. Samples collected every 12 hr (Rio) or every 24 hr (Camden) were extracted sequentially with cyclohexane (CX), dichloromethane (DCM), and acetone (ACE), for a rough fractionation by polarity, and composites of the extracts were tested for mutagenicity using the Salmonella frame shift (TA98) and base substitution (TA100) tester strains, as well as for genotoxicity using the Rossman Microscreen bioassay based on the induction of lambda-prophage in a lysogenic Escherichia coli strain. All samples were tested without and with S9 metabolic activation. Maximum mutagenic and genotoxic activities were in the nonpolar (CX) and polar (ACE) fractions, respectively, indicating that these two assays detect different classes of compounds with different efficiencies. Oxidative aging of the Rio aerosol is indicated by a shift in activities in both tests from the less polar fractions in the day to the polar (ACE) fraction at night. The Rio TA98 mutagenic (18 rev/m3) and genotoxic (1.4 x 10(5) PFU/m3) activities were higher than those for Camden, an Eastern U.S. city, by factors of 1.4 and 2.8, respectively.
Authors:
A G Miguel; J M Daisey; J A Sousa
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Environmental and molecular mutagenesis     Volume:  15     ISSN:  0893-6692     ISO Abbreviation:  Environ. Mol. Mutagen.     Publication Date:  1990  
Date Detail:
Created Date:  1990-02-23     Completed Date:  1990-02-23     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8800109     Medline TA:  Environ Mol Mutagen     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  36-43     Citation Subset:  IM    
Affiliation:
Biophysics Institute, Federal University of Rio de Janeiro, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Air Pollutants / toxicity*
Animals
Bacteriophage lambda / drug effects
Biotransformation
Brazil
Male
Mutagenicity Tests / methods
Mutagens*
Rats
Salmonella typhimurium / drug effects
Solvents
Time Factors
United States
Virus Activation / drug effects
Grant Support
ID/Acronym/Agency:
ES 00260/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Air Pollutants; 0/Mutagens; 0/Solvents

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