Document Detail

Comparative study on metallothionein induction in whole testicular tissue and isolated Leydig cells.
MedLine Citation:
PMID:  2053849     Owner:  NLM     Status:  MEDLINE    
The present study aimed at investigating whether previously reported interstrain differences of mice in testicular susceptibility to cadmium toxicity can be attributed to different metallothionein expression in the testes and other organs. Furthermore, purified Leydig cell systems were employed to identify a testicular compartment capable to respond specifically to exogenous cadmium by increased metallothionein expression. It was shown that species differences in testicular damage after cadmium administration can not be explained by differences in cadmium-induced stimulation of hepatic and renal metallothionein. In contrast to these organs, no metallothionein response to cadmium was observed in testicular homogenates. However, both freshly isolated and purified rat Leydig cells and a murine tumor Leydig cell line are not only able to synthesize metallothionein under basal conditions, but also respond to exogenous cadmium by increased synthesis of metallothionein (35S-cysteine incorporation) and an increased Mt-mRNA content. Extracts from freshly isolated and purified rat Leydig cells contained a cadmium-binding component which eluted in gel chromatography with the same properties as metallothionein. It was concluded that the use of purified cell systems is a valuable tool to investigate possible targets of cadmium toxicity.
J Abel; N de Ruiter; W N Kühn-Velten
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Archives of toxicology     Volume:  65     ISSN:  0340-5761     ISO Abbreviation:  Arch. Toxicol.     Publication Date:  1991  
Date Detail:
Created Date:  1991-07-23     Completed Date:  1991-07-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0417615     Medline TA:  Arch Toxicol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  228-34     Citation Subset:  IM    
Abteilung Toxikologie, Heinrich-Heine-Universität, Düsseldorf, Federal Republic of Germany.
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MeSH Terms
Cadmium / metabolism,  toxicity*
Cell Line
Keratinocytes / metabolism
Kidney / metabolism
Leydig Cells / drug effects,  metabolism*
Liver / metabolism
Metallothionein / analysis,  biosynthesis*,  genetics
Mice, Inbred C57BL
Mice, Inbred DBA
RNA, Messenger / analysis
Species Specificity
Testis / drug effects,  metabolism*
Zinc / pharmacology
Reg. No./Substance:
0/RNA, Messenger; 0/cadmium-binding protein; 7440-43-9/Cadmium; 7440-66-6/Zinc; 9038-94-2/Metallothionein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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