Document Detail


Comparative study of indirect immunofluorescence and immunoblotting for the diagnosis of autoimmune pemphigus.
MedLine Citation:
PMID:  7979544     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The diagnosis of pemphigus relies on immunopathological criteria including the detection of circulating autoantibodies to desmosomal components. In the present work we compared the usefulness of immunoblotting (IB) and indirect immunofluorescence (IIF) in the diagnosis of pemphigus using monkey oesophagus (MO) and rabbit lip (RL) as epithelial substrates. Among 54 sera from patients with well-documented pemphigus (40 pemphigus vulgaris, PV, and 14 pemphigus foliaceus, PF), 46 (85%) proved positive by IIF (46 on MO and 41 on RL) as compared with 44 (81.5%) positive by IB. IIF and IB were equally sensitive (90%) for the diagnosis of PV whereas IIF (on RL) was more sensitive (71%) than IB (57%) for the detection of PF autoantibodies. However, when the two techniques were considered in combination, the sensitivity of the detection of pemphigus autoantibodies rose to 94.5%. An IB study would therefore be warranted in the presence of an (alleged) pemphigus serum that was IIF-negative since approximately 10% of these were found to be positive. Furthermore, the pattern of IB reactivity may assist in classification, since the 130- and the 160-kDa antigens seem specifically correlated with PV and PF, respectively.
Authors:
E Cozzani; J Kanitakis; J F Nicolas; D Schmitt; J Thivolet
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Archives of dermatological research     Volume:  286     ISSN:  0340-3696     ISO Abbreviation:  Arch. Dermatol. Res.     Publication Date:  1994  
Date Detail:
Created Date:  1994-12-19     Completed Date:  1994-12-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8000462     Medline TA:  Arch Dermatol Res     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  295-9     Citation Subset:  IM    
Affiliation:
Laboratory of Dermatopathology/Department of Dermatology, Hôpital Edouard Herriot, Lyon, France.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Animals
Autoantibodies / blood
Fluorescent Antibody Technique
Haplorhini
Humans
Immunoblotting
Infant
Male
Middle Aged
Pemphigus / diagnosis*,  immunology
Rabbits
Retrospective Studies
Chemical
Reg. No./Substance:
0/Autoantibodies
Comments/Corrections
Erratum In:
Arch Dermatol Res 1994;286(8):507

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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