Document Detail


Comparative study of in vitro digestibility of major allergen tropomyosin and other food proteins of Chinese mitten crab (Eriocheir sinensis).
MedLine Citation:
PMID:  20564455     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: China is the largest producer and consumer of aquatic products in the world; however, many people in China suffer from allergies upon consuming crab. Stability in simulated gastric fluid is regarded as an important parameter for the estimation of food allergenicity.
RESULTS: The digestive stability of allergenic protein tropomyosin (TM) and other food proteins from Chinese mitten crab in simulated gastric fluid (SGF) and simulated intestinal fluid (SIF) digestion assay systems was investigated and compared by SDS-PAGE, western blot and inhibition ELISA. In the SGF system, proteins such as the original band of myosin heavy chain (MHC) and actin were rapidly degraded within a short period of time, while TM was relatively resistant to pepsin digestion. In the SIF system, MHC was easily decomposed, while TM and actin were similarly resistant to digestion. Further study by IgE immunoblotting and inhibition ELISA using sera from crab-allergic patients indicated that allergenicity of TM was partially decreased.
CONCLUSION: Chinese mitten crab major allergen TM was resistant to pepsin while relatively susceptible to trypsin and chymotrypsin digestion. Both SDS-PAGE using purified TM and western blot using myofibrillar proteins indicated that the degradation pattern of TM by SGF and SIF was not affected by the presence of other myofibrillar proteins. Inhibition ELISA results revealed that proteinase digestion is effective in reducing the allergenicity of crab TM.
Authors:
Guang-Ming Liu; Min-Jie Cao; Yuan-Yuan Huang; Qiu-Feng Cai; Wu-Yin Weng; Wen-Jin Su
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of the science of food and agriculture     Volume:  90     ISSN:  1097-0010     ISO Abbreviation:  J. Sci. Food Agric.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-06-24     Completed Date:  2011-01-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376334     Medline TA:  J Sci Food Agric     Country:  England    
Other Details:
Languages:  eng     Pagination:  1614-20     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 Society of Chemical Industry.
Affiliation:
College of Biological Engineering, The Key Laboratory of Science and Technology for Aquaculture and Food Safety, Jimei University, 43 Yindou Road, Xiamen, Fujian, China. gmliu@jmu.edu.cn
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism
Adolescent
Adult
Animals
Blotting, Western
Brachyura / chemistry*
China
Digestion*
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Food Hypersensitivity / immunology,  metabolism,  prevention & control*
Gastric Juice
Humans
Immunoglobulin E / metabolism
Intestines
Pepsin A / metabolism
Proteins / immunology,  metabolism*
Tropomyosin / immunology,  metabolism*
Young Adult
Chemical
Reg. No./Substance:
0/Actins; 0/Proteins; 0/Tropomyosin; 37341-29-0/Immunoglobulin E; EC 3.4.23.1/Pepsin A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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