| Comparative study of the dynamics of focal contacts in live epithelial and mesenchymal cells. | |
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MedLine Citation:
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PMID: 21424934 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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To further characterize the morphology and dynamics of focal contacts (FCs) in epithelial cells, we compared the size, number, localization, velocity, and turnover of FCs in epithelial and mesenchymal cell lines. Using immunocytochemistry, we found there were no significant differences between mesenchymal and epithelial cells in number and appearance whereas the location and size of FCs in each cell were different between mesenchymal and epithelial cells. FCs in mesenchymal cells localized at the cell periphery and cell center, but FCs were found only at the cell periphery in epithelial cells. The size of FCs in epithelial cells were significantly smaller than in mesenchymal cells. Next, we compared the dynamics of FCs in both mesenchymal and epithelial cells and found no significant difference between the two groups. Finally, we added inhibitors for the hemidesmosome (HD) proteins, α6 integrin and β4 integrin, to HaCat cell (epithelial) cultures and examined the number and size of FCs. Under these conditions, the size and localization of FCs in HaCat cells became comparable to that of mesenchymal cells. Therefore, we concluded the size and localization of FCs is regulated by the existence of HDs in epithelial cells. |
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Authors:
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Toshiyuki Ozawa; Daisuke Tsuruta |
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Publication Detail:
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Type: Journal Article Date: 2011-03-23 |
Journal Detail:
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Title: Medical molecular morphology Volume: 44 ISSN: 1860-1499 ISO Abbreviation: Med Mol Morphol Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-03-22 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101239023 Medline TA: Med Mol Morphol Country: Japan |
Other Details:
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Languages: eng Pagination: 27-33 Citation Subset: IM |
Affiliation:
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Department of Dermatology, Osaka City University Graduate School of Medicine, 1-4-3 Asahimachi, Abeno-ku, Osaka, 545-8585, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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