Document Detail


Comparative study of colorimetric DNA hybridization method and conventional culture procedure for detection of Salmonella in foods.
MedLine Citation:
PMID:  2376546     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A second generation nucleic acid hybridization assay has been developed and evaluated against the conventional culture method for detection of salmonellae in foods. The assay involves a liquid hybridization with Salmonella-specific oligonucleotide probes, capture of probe:target hybrids onto a solid support (plastic dipstick), and a colorimetric end point detection. The assay can be completed in 2.5 h, following approximately 44 h of culture enrichment. One thousand samples representing 20 food types were analyzed in parallel by both methods. Samples included uninoculated test product, and product inoculated with Salmonella at 2 levels. Eighteen Salmonella serotypes were used as inocula. The data demonstrate that the colorimetric hybridization method and the conventional culture method are equivalent in their ability to detect Salmonella contamination of foods.
Authors:
S W Chan; S G Wilson; M Vera-Garcia; K Whippie; M Ottaviani; A Whilby; A Shah; A Johnson; M A Mozola; D N Halbert
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal - Association of Official Analytical Chemists     Volume:  73     ISSN:  0004-5756     ISO Abbreviation:  J Assoc Off Anal Chem     Publication Date:    1990 May-Jun
Date Detail:
Created Date:  1990-09-04     Completed Date:  1990-09-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7505559     Medline TA:  J Assoc Off Anal Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  419-24     Citation Subset:  IM    
Affiliation:
Gene-Trak Systems, Framingham, MA 01701.
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MeSH Terms
Descriptor/Qualifier:
Animals
Colorimetry
DNA, Bacterial / analysis*
Food Contamination / analysis*
Food Microbiology*
Nucleic Acid Hybridization
Salmonella / isolation & purification*
Chemical
Reg. No./Substance:
0/DNA, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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