Document Detail

Comparative study of Cyperus rotundus essential oil by a modified GC/MS analysis method. Evaluation of its antioxidant, cytotoxic, and apoptotic effects.
MedLine Citation:
PMID:  18493959     Owner:  NLM     Status:  MEDLINE    
Gas chromatography coupled with mass spectrometry (GC/MS), using both electron impact (EI) and chemical ionization (CI) detection modes on apolar and polar stationary phases, led to the determination of the volatile composition of the essential oil obtained from tubers of Cyperus rotundus (Cyperaceae). In this study, more than 33 compounds were identified and then compared with the results obtained in our previous work. Cyperene, alpha-cyperone, isolongifolen-5-one, rotundene, and cyperorotundene were the principal compounds comprising 62% of the oil. An in vitro cytotoxicity assay with MTT indicated that this oil was very effective against L1210 leukaemia cells line. This result correlates with significantly increased apoptotic DNA fragmentation. The oxidative effects of the essential oil were evaluated using the 1,1-diphenyl-2-picrylhydrazyl (DPPH), xanthine/xanthine oxidase assays, and the scavenging of superoxide radical assay generated by photo-reduction of riboflavin. The antimutagenic activity of essential oil has been examined by following the inhibition of H(2)O(2) UV photolysis which induced strand-break formation in pBS plasmid DNA scission assay. Based on all these results, it is concluded that C. rotundus essential-oil composition established by GC/MS analysis, in EI- and CI-MS modes, presents a variety of a chemical composition we were not able to detect with only GC/MS analysis in our previous work. This essential oil exhibited antioxidant, cytotoxic, and apoptotic properties.
Soumaya Kilani; Jérôme Ledauphin; Ines Bouhlel; Mohamed Ben Sghaier; Jihed Boubaker; Inès Skandrani; Ridha Mosrati; Kamel Ghedira; Daniel Barillier; Leila Chekir-Ghedira
Related Documents :
23401009 - An immobilized graphene oxide stationary phase for open-tubular capillary electrochroma...
24375639 - Application of direct hptlc-maldi for the qualitative and quantitative profiling of neu...
24312769 - Application of lactobionic acid and nonionic surfactants as solubilizing agents for par...
18274279 - Bioactive compounds of the volatile oil of dracocephalum kotschyi.
20395139 - An analysis of the microwave dielectric properties of solvent-oil feedstock mixtures at...
20015509 - Gas chromatographic-mass spectrometric analysis of volatiles obtained by four different...
20629389 - Validated stability-indicating rp-hplc method for the determination of rimonabant in a ...
9839189 - Semiquantitative bioluminescent assay of glutathione.
8807549 - Method development for the determination of cefdinir and its related substances by high...
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Chemistry & biodiversity     Volume:  5     ISSN:  1612-1880     ISO Abbreviation:  Chem. Biodivers.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-05-26     Completed Date:  2008-08-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101197449     Medline TA:  Chem Biodivers     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  729-42     Citation Subset:  IM    
Unité de Pharmacognosie/Biologie Moléculaire 99/UR/07-03, Faculty of Pharmacy, Monastir 5000, Tunisia.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Antioxidants / chemistry*,  pharmacology*
Apoptosis / drug effects*
Cell Line, Tumor
Cell Proliferation / drug effects
Cyperus / chemistry*
Free Radicals / metabolism
Gas Chromatography-Mass Spectrometry / methods*
Hydrogen Peroxide / pharmacology
Molecular Structure
Oils, Volatile / chemistry*,  pharmacology*
Plant Extracts / chemistry
Plasmids / genetics
Xanthine Oxidase / metabolism
Reg. No./Substance:
0/Antioxidants; 0/Free Radicals; 0/Oils, Volatile; 0/Plant Extracts; 7722-84-1/Hydrogen Peroxide; EC Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Quinolizidinyl-benzimidazoles as platelet-antiaggregating agents.
Next Document:  Chemical constituents of Stereum subtomentosum and two other birch-associated basidiomycetes: an int...