Document Detail


Comparative study of Bacillus thuringiensis Cry1Ia and Cry1Aa delta-endotoxins: activation process and toxicity against Prays oleae.
MedLine Citation:
PMID:  20123104     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cry1Ia and Cry1Aa proteins exhibited toxicities against Prays oleae with LC(50) of 189 and 116 ng/cm(2), respectively. The ability to process Cry1Ia11 protoxin by trypsin, chymotrypsin and P. oleae larvae proteases was studied and compared to that of Cry1Aa11. After solubilization under high alkaline condition (50mM NaOH), Cry1Aa11 was converted into a major fragment of 65 kDa, whereas Cry1Ia11 protoxin was completely degraded by P. oleae larvae proteases and trypsin and converted into a major fragment of 70 kDa by chymotrypsin. Using less proteases of P. oleae juice, the degradation of Cry1Ia11 was attenuated. When the solubilization (in 50mM Na(2)CO(3) pH 10.5 buffer) and activation were combined, Cry1Ia11 was converted into a proteolytic product of 70 kDa after 3h of incubation with trypsin, chymotrypsin and P. oleae juice. These results suggest that the in vivo solubilization of Cry1Ia11 was assured by larval proteases after a swelling of the corresponding inclusion due to the alkalinity of the larval midgut.
Authors:
Mariam Dammak; Slim Tounsi; Souad Rouis; Samir Jaoua
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-02-01
Journal Detail:
Title:  Journal of invertebrate pathology     Volume:  104     ISSN:  1096-0805     ISO Abbreviation:  J. Invertebr. Pathol.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-13     Completed Date:  2010-07-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0014067     Medline TA:  J Invertebr Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  39-43     Citation Subset:  IM    
Copyright Information:
(c) 2010 Elsevier Inc. All rights reserved.
Affiliation:
Laboratory of Biopesticides, Centre of Biotechnology of Sfax, Tunisia, P.O. Box 1177, 3061 Sfax, Tunisia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Proteins / metabolism*,  toxicity*
Blotting, Western
Electrophoresis, Polyacrylamide Gel
Endotoxins / metabolism*,  toxicity*
Hemolysin Proteins / metabolism*,  toxicity*
Moths / metabolism*,  parasitology*
Recombinant Proteins / metabolism,  toxicity
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Endotoxins; 0/Hemolysin Proteins; 0/Recombinant Proteins; 0/insecticidal crystal protein, Bacillus Thuringiensis

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