Document Detail

Comparative studies of total cross-linking, cell survival and cell cycle perturbations in Chinese hamster cells treated with alkylating agents in vitro.
MedLine Citation:
PMID:  3516154     Owner:  NLM     Status:  MEDLINE    
The toxicities of 4-hydroperoxycyclophosphamide (4-OOH CY), phosphoramide mustard (PM), melphalan (MEL) and busulphan (BU) have been compared in Chinese hamster cells, V-79-753B. The initial total amount of cross-linking was a determining factor for the clonogenic survival of cells treated with MEL or PM. Although 4-OOH CY generated cross-links in this cell line, this damage did not account for the toxicity of the compound. There was no evidence for cross-link formation in cells treated with BU, even at a dose of the drug (1000 micrograms/ml) that was too toxic to measure clonogenic survival. Comparison for the four compounds at equitoxic doses showed that both PM and MEL caused the arrest of the cell cycle at G2 which persisted after drug removal. This was accompanied by a decline in the population growth rate and a decrease in total cell count. In contrast, both BU and 4-OOH CY caused a temporary arrest of the cell cycle G2, 24 hr after drug removal. However, the cell cycle distribution returned the control values within 3-4 days after treatment. Both BU and 4-OOH CY showed little effect on the initial growth rate of the cells. It is concluded that the initial amount of cross-links contributes to the toxicity of PM and MEL. However, it is unlikely that the generation of cross-links is of major importance for the toxicity of either 4-OOH CY or BU.
B C Millar; M J Tilby; M G Ormerod; A W Payne; S Jinks; P S Loverock
Related Documents :
3654334 - The effect of hydrocortisone on the para-aortic body of the newborn mouse: an in vivo f...
2328724 - Translocation of bfgf to the nucleus is g1 phase cell cycle specific in bovine aortic e...
1176544 - Effect of near ultraviolet and visible light on amoeba.
7116934 - Cytogenetic and flow cytometric studies of cells from patients with fanconi's anemia.
3180064 - Nuclear fragmentation and premature chromosome condensation induced by heat shock in s-...
8886534 - The non-langerhans' cell histiocytoses in childhood.
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical pharmacology     Volume:  35     ISSN:  0006-2952     ISO Abbreviation:  Biochem. Pharmacol.     Publication Date:  1986 Apr 
Date Detail:
Created Date:  1986-05-09     Completed Date:  1986-05-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0101032     Medline TA:  Biochem Pharmacol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1163-9     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Alkylating Agents / toxicity*
Busulfan / toxicity
Cell Cycle / drug effects
Cell Line
Cell Survival / drug effects
Cyclophosphamide / analogs & derivatives,  toxicity
Dose-Response Relationship, Drug
Melphalan / toxicity
Ovary / cytology
Phosphoramide Mustards / toxicity
Reg. No./Substance:
0/Alkylating Agents; 0/Phosphoramide Mustards; 10159-53-2/phosphoramide mustard; 148-82-3/Melphalan; 39800-16-3/perfosfamide; 50-18-0/Cyclophosphamide; 55-98-1/Busulfan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Purification and characterization of bacteriophage 9NA lysozyme.
Next Document:  Mercapturic acid formation is an activation and intermediary step in the metabolism of hexachlorobut...