Document Detail


Comparative studies of thyroxine binding proteins in turtle plasma using column chromatography.
MedLine Citation:
PMID:  8428644     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Thyroxine (T4) binding proteins in turtle plasma were compared with those of various mammalian and nonmammalian (fish, birds) species using gel filtration chromatography (Bio-Gel). High-affinity T4 binding was observed in all mammals studied, in the chicken, and in one species of turtle, Trachemys scripta. This high affinity T4 binding protein (TBP) appears to be the major component of T4 transport in the turtle; selective removal of this TBP greatly diminished binding activity in T. scripta plasma. In all other nonmammalian species studied (including other turtles), T4 binding appeared to be associated primarily with relatively low affinity components such as albumin. Albumin concentrations in turtle (T. scripta, and Chelydra serpentina) plasmas were examined by measurement of dry weight, protein assay (Bradford), radioimmunoassay, and electrophoretic densitometry. While C. serpentina may have total protein concentrations approaching that of human plasma, albumin content in both turtles was estimated at about 3-4 mg/ml, less than 10% that of human plasma albumin; larger molecular weight components that do not bind T4 comprise a greater proportion of turtle plasma protein. Additionally, purified T. scripta albumin exhibits lower T4 binding than does human albumin; binding to turtle albumin is also reduced by fatty acids. Taken together, these results indicate a lesser role for albumin in plasma T4 binding in T. scripta than in humans, and, thus, a larger role for the T4 binding protein, TBP. Other turtles lacking a TBP may rely wholly on low affinity albumin binding that also has a lower capacity than that in mammals.
Authors:
K A Glennemeier; P Licht
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  General and comparative endocrinology     Volume:  89     ISSN:  0016-6480     ISO Abbreviation:  Gen. Comp. Endocrinol.     Publication Date:  1993 Jan 
Date Detail:
Created Date:  1993-03-11     Completed Date:  1993-03-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370735     Medline TA:  Gen Comp Endocrinol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  119-31     Citation Subset:  IM    
Affiliation:
Department of Integrative Biology, University of California, Berkeley 94720.
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MeSH Terms
Descriptor/Qualifier:
Adult
Animals
Chickens / blood
Chromatography, Gel*
Female
Fishes / blood
Humans
Iodine Radioisotopes
Serum Albumin / metabolism
Thyroglobulin / metabolism
Thyroxine / blood
Thyroxine-Binding Proteins / metabolism*
Turtles / blood*
Chemical
Reg. No./Substance:
0/Iodine Radioisotopes; 0/Serum Albumin; 0/Thyroxine-Binding Proteins; 7488-70-2/Thyroxine; 9010-34-8/Thyroglobulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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