Document Detail


Comparative studies of thiol-sensitive fluorogenic probes for HAT assays.
MedLine Citation:
PMID:  23138472     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Histone acetyltransferases (HATs) catalyze the acetylation of specific lysine residues in histone and nonhistone proteins. Recent studies showed that acetylation is widely distributed among cellular proteins, suggestive of diverse functions of HATs in cellular pathways. Nevertheless, currently available assays for HAT activity study are still quite limited. Here, we evaluated a series of thiol-sensitive fluorogenic compounds for the detection of the enzymatic activities of different HAT proteins. Upon conjugation to the thiol group of HSCoA, these molecules gain enhanced quantum yields and strong fluorescence, permitting facile quantitation of HAT activities. We investigated and compared the assay performances of these fluorogenic compounds for their capability as HAT activity reporters, including kinetics of reaction with HSCoA, influence on HAT activity, and fluorescence amplification factors. Our data suggest that CPM and coumarin maleic acid ester are excellent HAT probes owing to their fast reaction kinetics and dramatic fluorescence enhancement during the HAT reaction. Further, the microtiter plate measurements show that this fluorescent approach is robust and well suited for adaption to high-throughput screening of small molecule inhibitors of HATs, highlighting the value of this assay strategy in new drug discovery.
Authors:
Tielong Gao; Chao Yang; Yujun George Zheng
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-11-09
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  405     ISSN:  1618-2650     ISO Abbreviation:  Anal Bioanal Chem     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-18     Completed Date:  2013-06-24     Revised Date:  2014-07-14    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1361-71     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Enzyme Assays / methods*
Fluorescent Dyes / chemistry*
Histone Acetyltransferases / chemistry*
Humans
Kinetics
Sulfhydryl Compounds / analysis
Grant Support
ID/Acronym/Agency:
R01 GM086717/GM/NIGMS NIH HHS; R01GM086717/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 0/Sulfhydryl Compounds; EC 2.3.1.48/Histone Acetyltransferases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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