| Comparative studies of skeletal muscle proteome and transcriptome profilings between pig breeds. | |
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MedLine Citation:
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PMID: 20532784 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Two genetically different pig breeds, the Korean native pig (KNP) and the Western meat-producing Landrace, show breed-specific traits in stress responsiveness (stress hormone levels), growth performance (live weight), and meat quality (intramuscular fat content). We analyzed expression levels within the proteome and transcriptome of the longissimus muscles of both breeds using two-dimensional electrophoresis (2-DE) and microarray analysis. We constructed a porcine proteome database focused mainly on mitochondrial proteins. In total, 101 proteins were identified, of which approximately 60% were metabolic enzymes and mitochondrial proteins. We screened several proteins and genes related to stress and metabolism in skeletal muscles using comparative analysis. In particular, three stress-related genes (heat shock protein beta-1, stress-70 protein, and heat shock 70 kDa protein) were more highly expressed in the Landrace than in the KNP breed. Six metabolism-related genes (peroxisome proliferative activated receptor alpha, short-chain acyl-CoA dehydrogenase, succinate dehydrogenase, NADH-ubiquinone oxidoreductase, glycerol-3-phosphate dehydrogenase, and sterol regulatory element binding protein-1c), all of which are involved in energy and lipid metabolism, were more highly expressed at the protein or mRNA level in the KNP breed. These data may reflect the breed dependence of traits such as stress responsiveness, growth performance, and meat quality. |
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Authors:
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Nam-Kuk Kim; Hye-Ran Park; Hwi-Cheul Lee; Duhak Yoon; Eun-Suk Son; Yeun-Song Kim; Se-Ra Kim; Oun-Hyun Kim; Chang-Soo Lee |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-08 |
Journal Detail:
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Title: Mammalian genome : official journal of the International Mammalian Genome Society Volume: 21 ISSN: 1432-1777 ISO Abbreviation: Mamm. Genome Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-06-24 Completed Date: 2010-10-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9100916 Medline TA: Mamm Genome Country: United States |
Other Details:
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Languages: eng Pagination: 307-19 Citation Subset: IM |
Affiliation:
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Department of Applied Biochemistry, College of Biomedical & Health Science, Konkuk University, 322 Danwol-dong, Chungju, Republic of Korea. nkvirus@korea.kr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Breeding Gene Expression Profiling* Lipid Metabolism Meat Muscle, Skeletal / metabolism* Proteome / analysis* RNA, Messenger / metabolism Sus scrofa / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Proteome; 0/RNA, Messenger |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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