Document Detail


Comparative studies on membrane-associated, folded chromosomes from Escherichia coli.
MedLine Citation:
PMID:  770443     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nuclear bodies were isolated from Escherichia coli spheroplasts by two different lysis procedures. Their lipid and protein content and the superhelix density was measured. The preparations differed mainly in respect to the amount of the attached membrane material, which seems to be an essential factor in maintaining the stability of the nuclear bodies. The superhelix density was found to be higher than that of naturally occurring, covalently closed, circular deoxyribonucleic acids. The influence of the preparative method on the superhelicity was comparatively small.
Authors:
P Dworsky
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  126     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1976 Apr 
Date Detail:
Created Date:  1976-07-06     Completed Date:  1976-07-06     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  64-71     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / analysis
Bacteriolysis
Cetrimonium Compounds / pharmacology
Chromosomes, Bacterial / analysis*
DNA, Bacterial / analysis*,  isolation & purification
Deoxycholic Acid / pharmacology
Escherichia coli / analysis*,  drug effects
Lipids / analysis
Muramidase / pharmacology
Rifampin / pharmacology
Spheroplasts / analysis,  drug effects
Surface-Active Agents / pharmacology
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Cetrimonium Compounds; 0/DNA, Bacterial; 0/Lipids; 0/Surface-Active Agents; 13292-46-1/Rifampin; 83-44-3/Deoxycholic Acid; EC 3.2.1.17/Muramidase
Comments/Corrections

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