| Comparative studies on the extraction of metagenomic DNA from the saline habitats of Coastal Gujarat and Sambhar Lake, Rajasthan (India) in prospect of molecular diversity and search for novel biocatalysts. | |
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MedLine Citation:
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PMID: 20600268 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Extraction of total DNA from a given habitat assumes significance in metagenomics, due to the requirement of inhibitor free and high quality metagenome in good quantity for applications in molecular biology. DNA extraction and its quality assessment for PCR applications from saline soils of Coastal Gujarat and Sambhar Soda Lake, Rajasthan in India is described in a comparative manner. The mechanical and soft lysis methods were simple and efficient for rapid isolation of PCR amplifiable total genomic DNA. The results are significant as only few extreme environments, particularly saline habitats are explored for their metagenomic potential. |
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Authors:
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P K Siddhapura; S Vanparia; M K Purohit; S P Singh |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-23 |
Journal Detail:
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Title: International journal of biological macromolecules Volume: 47 ISSN: 1879-0003 ISO Abbreviation: Int. J. Biol. Macromol. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-08-06 Completed Date: 2011-01-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7909578 Medline TA: Int J Biol Macromol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 375-9 Citation Subset: IM |
Affiliation:
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Department of Biosciences, Saurashtra University, Rajkot, Gujarat, India. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Proteins
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genetics Biocatalysis* Biodiversity* DNA / genetics, isolation & purification* Ecosystem* Electrophoresis, Agar Gel Endopeptidases / genetics Fresh Water* India Metagenomics* Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S / genetics, isolation & purification Spectrophotometry |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/RNA, Ribosomal, 16S; 9007-49-2/DNA; EC 3.4.-/Endopeptidases; EC 3.4.99.-/alkaline protease |
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