Document Detail


Comparative studies on the effects of specific immunoneutralization of endogenous FSH or LH on testicular germ cell transformations in the adult bonnet monkey (Macaca radiata).
MedLine Citation:
PMID:  7576129     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PROBLEM: It is yet to be determined clearly whether the two hormones FSH and T act synergistically in the same cell type--the Sertoli cells--to control overall spermatogenesis or influence independently the transformation of specific germ cell types during spermatogenesis in the adult mammal. METHOD: Adult male bonnet monkeys specifically deprived of either FSH or LH using immunoneutralization techniques were monitored for changes in testicular germ cell transformation by DNA flow cytometry. RESULTS: FSH deprivation caused a significant reduction ( > 40%; P < 0.05) in [3H] thymidine incorporation into DNA of proliferating 2C (spermatogonial) cells, a marked inhibition ( > 50%) in the transformation of 2C to primary spermatocytes (4C) and a concomitant, belated reduction (50%) in the formation of round spermatids (1C). In contrast, specific LH/T deprivation led to an immediate arrest in the meiotic transformation of 4C to 1C/HC leading to an effective and significant block ( < 90%; P < 0.01) in sperm production. CONCLUSION: Thus, LH rather than FSH deprivation has a more pronounced and immediate effect as the former primarily blocks meiosis (4C --> 1C/HC) which controls production of spermatids. These data provide evidence for LH/T and FSH regulating spermatogenic process in the adult primate by primarily acting at specific germ cell transformation steps.
Authors:
R Suresh; R Medhamurthy; N R Moudgal
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  American journal of reproductive immunology (New York, N.Y. : 1989)     Volume:  34     ISSN:  1046-7408     ISO Abbreviation:  Am. J. Reprod. Immunol.     Publication Date:  1995 Jul 
Date Detail:
Created Date:  1995-12-28     Completed Date:  1995-12-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8912860     Medline TA:  Am J Reprod Immunol     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  35-43     Citation Subset:  IM    
Affiliation:
Primate Research Laboratory, Center for Reproductive Biology and Molecular Endocrinology, Indian Institute of Science, Bangalore.
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MeSH Terms
Descriptor/Qualifier:
Animals
Flow Cytometry
Follicle Stimulating Hormone / blood,  immunology*
Immune Sera / pharmacology
Luteinizing Hormone / blood,  immunology*
Macaca radiata
Male
Sertoli Cells / immunology
Spermatogenesis / immunology*
Testis / cytology,  immunology*,  physiology
Testosterone / blood,  immunology*
Chemical
Reg. No./Substance:
0/Immune Sera; 58-22-0/Testosterone; 9002-67-9/Luteinizing Hormone; 9002-68-0/Follicle Stimulating Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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