Document Detail

Comparative studies on the effects of doxorubicin and differentiation inducing agents on B16 melanoma cells.
MedLine Citation:
PMID:  1326307     Owner:  NLM     Status:  MEDLINE    
The differentiation-inducing activity of doxorubicin on B16 melanoma cells grown in vitro was compared with that of other known differentiation inducers, such as theophylline, retinoic acid, and melanocyte-stimulating hormone (MSH). At drug concentrations resulting in cytostatic effects, doxorubicin and theophylline induced morphological changes (dendritic-like structures with a terminal melanin granule) with an enhancement of total melanin content and tyrosinase activity. Retinoic acid did not alter melanin content and cell morphology, although it affected cell growth. MSH enhanced total melanin content and tyrosinase activity, with no significant morphological changes. Flow cytometric analysis showed that MSH led to an accumulation of cells in G1 phase whereas doxorubicin induced an accumulation of cells in G2 + M. Studies on DNA content in doxorubicin-treated cells, selected on the basis of a morphologically differentiated pattern, showed a clustering of these cells in G2 + M, probably due to a cytokinesis block. Thus doxorubicin can induce cell differentiation comparable with other differentiation inducers.
R Supino; M Mariani; A Colombo; E Prosperi; A C Croce; G Bottiroli
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of cancer (Oxford, England : 1990)     Volume:  28A     ISSN:  0959-8049     ISO Abbreviation:  Eur. J. Cancer     Publication Date:  1992  
Date Detail:
Created Date:  1992-10-19     Completed Date:  1992-10-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9005373     Medline TA:  Eur J Cancer     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  778-83     Citation Subset:  IM    
Istituto Nazionale Tumori, Milan, Italy.
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MeSH Terms
Cell Cycle / drug effects
Cell Differentiation / drug effects
Cell Survival / drug effects
DNA, Neoplasm / analysis
Doxorubicin / pharmacology*
Extracellular Space / chemistry
Intracellular Fluid / chemistry
Melanins / analysis
Melanoma, Experimental / drug therapy*,  pathology
Monophenol Monooxygenase / metabolism
Theophylline / pharmacology
Tretinoin / pharmacology
Tumor Cells, Cultured
alpha-MSH / pharmacology
Reg. No./Substance:
0/DNA, Neoplasm; 0/Melanins; 23214-92-8/Doxorubicin; 302-79-4/Tretinoin; 58-55-9/Theophylline; 581-05-5/alpha-MSH; EC Monooxygenase

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