Document Detail


Comparative structural elucidation of the K18, K22, and K100 antigens of Escherichia coli as related ribosyl-ribitol phosphates.
MedLine Citation:
PMID:  3282653     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The structures of the capsular K18, K22, and K100 antigens of E. coli O23:K18:H15, O23:K22:H15, and O75:K100:H5, respectively, were elucidated by determination of composition, 1H-, 13C-, and 31P-n.m.r. spectroscopy, periodate oxidation, alkaline hydrolysis followed by incubation with alkaline phosphatase, and methylation analysis of the polymers and their neutral fragmentation products. The polymers are poly(ribosyl-ribitol phosphates) related to the capsular antigen of H. influenzae (Hib). The K22 antigen has the repeating unit -P-2)-beta-Rib-(1----2)-RibOH-(5-, and the K18 antigen has the same polymer chain with partial 3-O-acetylation of the ribose moiety. The K100 antigen consists of repeating units of -P-3)-beta-Rib-(1----2)-RibOH-(5- and seems to have a secondary structure different from that of the other antigens. Together with the Hib capsular antigens, the structure of which was reported as -P-3)-beta-Rib-(1----1)-RibOH-(5-, these capsular antigens represent a structurally related group of capsular polymers.
Authors:
M L Rodriguez; B Jann; K Jann
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Carbohydrate research     Volume:  173     ISSN:  0008-6215     ISO Abbreviation:  Carbohydr. Res.     Publication Date:  1988 Mar 
Date Detail:
Created Date:  1988-06-07     Completed Date:  1988-06-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0043535     Medline TA:  Carbohydr Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  243-53     Citation Subset:  IM    
Affiliation:
Max-Planck-Institut für Immunobiologie, Freiburg-Zähringen, F.R.G.
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MeSH Terms
Descriptor/Qualifier:
Carbohydrate Conformation
Carbohydrate Sequence
Enzyme-Linked Immunosorbent Assay
Escherichia coli / immunology*
Hemagglutination
Immunoelectrophoresis
Indicators and Reagents
Magnetic Resonance Spectroscopy
Mass Spectrometry
Methylation
Polysaccharides, Bacterial*
Chemical
Reg. No./Substance:
0/Indicators and Reagents; 0/Polysaccharides, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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