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Comparative sensitivity of four different cell lines for the isolation of Coxiella burnetii.
MedLine Citation:
PMID:  22681323     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Coxiella burnetii is an obligate intracellular bacterium that causes the disease Q fever. This is usually diagnosed by serology (IFA) and/or PCR detection of C. burnetii DNA. However, neither of these methods can determine the viability of the bacterium. Four different cell lines were compared for their ability to amplify very low numbers of viable C. burnetii. Two different isolates of C. burnetii were used. For the Henzerling isolate, DH82 (dog macrophage) cells were the most sensitive with an ID(50) (dose required to infect 50% of cell cultures) of 14.6 bacterial copies. For the Arandale isolate, Vero (monkey epithelial) cells were the most sensitive with an ID(50) of less than one bacterium in a 100μl inoculum. The Vero cell line appeared highly useful as vacuoles could be seen microscopically in unstained infected cells. The findings of this study favour the use of Vero and DH82 tissue culture cell lines for isolation and growth of C. burnetii in vitro. The other cell lines, XTC-2 and L929, were less suitable. © 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Authors:
Michelle G Lockhart; Aminul Islam; Stan G Fenwick; Stephen R Graves; John Stenos
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Publication Detail:
Type:  LETTER     Date:  2012-6-11
Journal Detail:
Title:  FEMS microbiology letters     Volume:  -     ISSN:  1574-6968     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Affiliation:
The Australian Rickettsial Reference Laboratory, Barwon Biomedical Research, The Geelong Hospital, Bellarine Street, Geelong, Victoria, Australia, 3220; Murdoch University, South Street, Murdoch, Western Australia, Australia, 6150.
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