Document Detail


Comparative rates of sialylation by recombinant trans-sialidase and inhibitor properties of synthetic oligosaccharides from Trypanosoma cruzi mucins-containing galactofuranose and galactopyranose.
MedLine Citation:
PMID:  17292612     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The mucin-like glycoproteins of Trypanosoma cruzi have novel O-linked oligosaccharides that are acceptors of sialic acid in the trans-sialidase (TcTS) reaction. The transference of sialic acid from host glycoconjugates to the mucins is involved in infection and pathogenesis. The O-linked chains may contain galactofuranose in addition to the acceptor galactopyranose units. Thus far, the galactofuranose form was found in the mucins of strains belonging to the less infective lineage. The acceptor properties of the chemically synthesized oligosaccharides were now studied in order to correlate their structure with the ability to act as substrates. Recombinant TcTS and sialyllactose as donor were used. The reactions were followed by HPAEC-PAD. The K(m) values were calculated for the free sugars, the sugar alditols and the benzyl glycosides. All the compounds showed to be good acceptors of sialic acid. Thus, the introduction of galactofuranose in the mucins of the strains of lineage 1 would not be responsible for the diminished virulence of the strains. The oligosaccharides and derivatives inhibited the transfer of sialic acid to the substrate N-acetyllactosamine with IC(50) values between 0.6 and 4 mM.
Authors:
Rosalía Agustí; M Eugenia Giorgi; Verónica M Mendoza; Carola Gallo-Rodriguez; Rosa M de Lederkremer
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-01-31
Journal Detail:
Title:  Bioorganic & medicinal chemistry     Volume:  15     ISSN:  0968-0896     ISO Abbreviation:  Bioorg. Med. Chem.     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-03-13     Completed Date:  2007-06-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9413298     Medline TA:  Bioorg Med Chem     Country:  England    
Other Details:
Languages:  eng     Pagination:  2611-6     Citation Subset:  IM    
Affiliation:
CIHIDECAR, Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón II, 1428 Buenos Aires, Argentina.
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MeSH Terms
Descriptor/Qualifier:
Amino Sugars / chemistry
Animals
Carbohydrate Sequence
Escherichia coli / metabolism
Galactose / chemistry*,  isolation & purification
Kinetics
Molecular Sequence Data
Mucins / chemistry*
Neuraminidase / antagonists & inhibitors,  metabolism*
Oligosaccharides / chemical synthesis*,  pharmacology*
Recombinant Proteins / chemistry
Sialic Acids / metabolism*
Trypanosoma cruzi / chemistry*
Chemical
Reg. No./Substance:
0/Amino Sugars; 0/Mucins; 0/Oligosaccharides; 0/Recombinant Proteins; 0/Sialic Acids; 26566-61-0/Galactose; 32181-59-2/N-acetyllactosamine; EC 3.2.1.18/Neuraminidase

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