| Comparative rates of sialylation by recombinant trans-sialidase and inhibitor properties of synthetic oligosaccharides from Trypanosoma cruzi mucins-containing galactofuranose and galactopyranose. | |
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MedLine Citation:
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PMID: 17292612 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The mucin-like glycoproteins of Trypanosoma cruzi have novel O-linked oligosaccharides that are acceptors of sialic acid in the trans-sialidase (TcTS) reaction. The transference of sialic acid from host glycoconjugates to the mucins is involved in infection and pathogenesis. The O-linked chains may contain galactofuranose in addition to the acceptor galactopyranose units. Thus far, the galactofuranose form was found in the mucins of strains belonging to the less infective lineage. The acceptor properties of the chemically synthesized oligosaccharides were now studied in order to correlate their structure with the ability to act as substrates. Recombinant TcTS and sialyllactose as donor were used. The reactions were followed by HPAEC-PAD. The K(m) values were calculated for the free sugars, the sugar alditols and the benzyl glycosides. All the compounds showed to be good acceptors of sialic acid. Thus, the introduction of galactofuranose in the mucins of the strains of lineage 1 would not be responsible for the diminished virulence of the strains. The oligosaccharides and derivatives inhibited the transfer of sialic acid to the substrate N-acetyllactosamine with IC(50) values between 0.6 and 4 mM. |
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Authors:
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Rosalía Agustí; M Eugenia Giorgi; Verónica M Mendoza; Carola Gallo-Rodriguez; Rosa M de Lederkremer |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2007-01-31 |
Journal Detail:
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Title: Bioorganic & medicinal chemistry Volume: 15 ISSN: 0968-0896 ISO Abbreviation: Bioorg. Med. Chem. Publication Date: 2007 Apr |
Date Detail:
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Created Date: 2007-03-13 Completed Date: 2007-06-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9413298 Medline TA: Bioorg Med Chem Country: England |
Other Details:
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Languages: eng Pagination: 2611-6 Citation Subset: IM |
Affiliation:
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CIHIDECAR, Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón II, 1428 Buenos Aires, Argentina. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Sugars
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chemistry Animals Carbohydrate Sequence Escherichia coli / metabolism Galactose / chemistry*, isolation & purification Kinetics Molecular Sequence Data Mucins / chemistry* Neuraminidase / antagonists & inhibitors, metabolism* Oligosaccharides / chemical synthesis*, pharmacology* Recombinant Proteins / chemistry Sialic Acids / metabolism* Trypanosoma cruzi / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Amino Sugars; 0/Mucins; 0/Oligosaccharides; 0/Recombinant Proteins; 0/Sialic Acids; 26566-61-0/Galactose; 32181-59-2/N-acetyllactosamine; EC 3.2.1.18/Neuraminidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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