Document Detail


Comparative proteomic analysis of mushroom cell wall proteins among the different developmental stages of Pleurotus tuber-regium.
MedLine Citation:
PMID:  22646718     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Cell wall proteins (CWPs) play a vital role in the development of the different morphological stages including mycelium, fruiting body and sclerotium in mushrooms which are important human food sources. Using fractionation by detergents and reducing agents, mushroom cell wall fractions from the different developmental stages of Pleurotus tuber-regium (PTR) were prepared. Using one-dimensional gel electrophoresis coupled with LC-MS, there were 103, 91 and 48 non-covalently linked CWPs identified in the cell wall fractions of the PTR mycelium, fruiting body and sclerotium, respectively. Comparing the CWPs in these cell wall fractions, 19 of them were in common and among which 17 belonged to the functional categories of translation, ribosomal structure and biogenesis. This is the first study to provide important biochemical insights into the different developmental stages of PTR mediated by CWPs and the CWPs identified in this study helped to explain the morphological changes of PTR mushrooms during its cultivation.
Authors:
Lei Chen; Bo-Bo Zhang; Peter C K Cheung
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-5-30
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  -     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-5-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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