| Comparative proteomic analysis of hypoxia-treated and untreated human leukemic U937 cells. | |
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MedLine Citation:
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PMID: 16622835 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We reported recently that moderate hypoxia and hypoxia-mimetic agents could induce growth arrest and differentiation of leukemic cells via the mediation of hypoxia-inducible factor 1 alpha (HIF-1alpha), but the exact molecular mechanisms remain largely unknown. In this study, human acute promonocytic leukemic U937 cells were incubated under 2% O2 or in 50 microM of the hypoxia mimetic agent cobalt chloride (CoCl2) and normal oxygen for 24 h, and their protein expression profiles were compared by 2-DE coupled with MALDI-TOF/TOF MS/MS. We identified 62 and 16 proteins that were significantly deregulated by hypoxia and CoCl2 treatment, respectively. These proteins were mainly involved in metabolism, gene expression regulation, signal transduction, cell proliferation, differentiation and apoptosis. As an example, N-myc downstream regulated gene 1 (NDRG1), a putative differentiation-related gene, was up-regulated in both 2% O2- and CoCl2-treated U937 cells. Moreover, enforced HIF-1alpha expression also elevated NDRG1 mRNA and protein in U937 cells. These data will provide some clues for understanding mechanisms by which leukemic cells response to hypoxia. |
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Authors:
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Yu-Hui Han; Li Xia; Li-Ping Song; Ying Zheng; Wen-Li Chen; Lei Zhang; Ying Huang; Guo-Qiang Chen; Li-Shun Wang |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Proteomics Volume: 6 ISSN: 1615-9853 ISO Abbreviation: Proteomics Publication Date: 2006 Jun |
Date Detail:
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Created Date: 2006-05-31 Completed Date: 2006-07-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 101092707 Medline TA: Proteomics Country: Germany |
Other Details:
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Languages: eng Pagination: 3262-74 Citation Subset: IM |
Affiliation:
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Department of Pathophysiology, Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Rui-Jin Hospital, Shanghai Jiao-Tong University School of Medicine, Shanghai, P. R. China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects,
physiology Cell Differentiation / drug effects, physiology Cell Hypoxia / drug effects, physiology Cell Proliferation / drug effects Cobalt / toxicity Electrophoresis, Gel, Two-Dimensional Gene Expression Regulation / drug effects, physiology* Humans Proteome* Signal Transduction / drug effects, physiology Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization U937 Cells |
| Chemical | |
Reg. No./Substance:
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0/Proteome; 7440-48-4/Cobalt; 7646-79-9/cobaltous chloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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